Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve capturing an image of an anatomical region comprising a target tissue and determining, based on the image and based on a reference image, that a current alignment of the image capture device is aligned with a predefined alignment. The determination is performed before initiating capturing of a time series of fluorescence medical images from fluorescence emission. In response to the determining that the current alignment is aligned with the predefined alignment, capturing of the time series of fluorescence medical images from the fluorescence emission is initiated.

Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve capturing an image of an anatomical region comprising a target tissue and determining, based on the image and based on a reference image, that a current alignment of the image capture device is aligned with a predefined alignment. The determination is performed before initiating capturing of a time series of fluorescence medical images from fluorescence emission. In response to the determining that the current alignment is aligned with the predefined alignment, capturing of the time series of fluorescence medical images from the fluorescence emission is initiated.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes retrieving a time series of angiography images of tissue of a subject, defining a plurality of calculation regions, generating a time-intensity curve for each respective calculation region, calculating a rank value for each respective calculation region based on one or more parameters derived from the time-intensity curve; and generating a viewable image in which on the image position of each calculation region an indication is provided of the calculated rank value for that calculation region. Also disclosed are methods and systems for generating first and second time-intensity curves for respective first and second calculation regions, calculating first and second rank values for the respective calculation regions based on first and second pluralities of parameters selected to approximate the respective time-intensity curves, and generating a spatial map of the first and second calculated rank values.

Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve providing a reference image of an anatomical region of the subject, the anatomical region comprising a target tissue, processing the reference image to generate an alignment reference image, displaying the alignment reference image concurrently with real-time video of the anatomical region, and aligning the real-time video with the alignment reference image to overlay the real-time video with the alignment reference image. Following such alignment, the subject may be imaged using, for example, fluorescence imaging, wherein the fluorescence imaging may be performed by an image acquisition assembly aligned in accordance with the alignment.

Abstract: Disclosed herein are systems and methods that can assess whether there is an issue with tissue perfusion, which can help a clinician better predict any surgical complications that may arise. Fluorescence images of the tissue of a subject can continuously be observed until a portion of the tissue that first perfused with blood containing one or more fluorescent agents is at peak fluorescence. Any areas of the tissue from the fluorescence images that remain dark can be further observed until these areas of concern show their first sign of fluorescence. The time it takes for these areas of concern to show their first signs of fluorescence since the first onset of fluorescence in the tissue can be referred to as the relative onset fluorescence delay. If the relative onset fluorescence delay time is greater than a predetermined threshold, the clinician can alter or change the surgical plan.

Abstract: A method for fluorescence imaging includes receiving first fluorescence imaging data, receiving a user input selecting a reference region associated with the first fluorescence imaging data, determining a reference fluorescence intensity level based on the reference region, receiving second fluorescence imaging data, determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level, and displaying an indication of the relative fluorescence intensity level.

Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve providing a reference image of an anatomical region of the subject, the anatomical region comprising a target tissue, processing the reference image to generate an alignment reference image, displaying the alignment reference image concurrently with real-time video of the anatomical region, and aligning the real-time video with the alignment reference image to overlay the real-time video with the alignment reference image. Following such alignment, the subject may be imaged using, for example, fluorescence imaging, wherein the fluorescence imaging may be performed by an image acquisition assembly aligned in accordance with the alignment.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes retrieving a time series of angiography images of tissue of a subject, defining a plurality of calculation regions, generating a time-intensity curve for each respective calculation region, calculating a rank value for each respective calculation region based on one or more parameters derived from the time-intensity curve; and generating a viewable image in which on the image position of each calculation region an indication is provided of the calculated rank value for that calculation region. Also disclosed are methods and systems for generating first and second time-intensity curves for respective first and second calculation regions, calculating first and second rank values for the respective calculation regions based on first and second pluralities of parameters selected to approximate the respective time-intensity curves, and generating a spatial map of the first and second calculated rank values.

Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve providing a reference image of an anatomical region of the subject, the anatomical region comprising a target tissue, processing the reference image to generate an alignment reference image, displaying the alignment reference image concurrently with real-time video of the anatomical region, and aligning the real-time video with the alignment reference image to overlay the real-time video with the alignment reference image. Following such alignment, the subject may be imaged using, for example, fluorescence imaging, wherein the fluorescence imaging may be performed by an image acquisition assembly aligned in accordance with the alignment.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes receiving a time series of fluorescence images of the tissue of the subject wherein the images define a plurality of calculation regions, generating a plurality of time-intensity curves for the plurality of calculation regions, creating a set of parameter values for each calculation region, generating a total rank value for each calculation region by comparing the sets of parameter values, and converting the total rank value into a ranking map image. Also disclosed are methods and systems for characterizing a wound in tissue by generating a wound index value.

Abstract: Systems and methods for storing data for a first time series of fluorescence images of the subject acquired during a first imaging session, storing data for a second time series of fluorescence images of the subject acquired during a second imaging session, receiving a request to view attributes of the subject, and in response to receiving the request, displaying a user interface on the display, the user interface comprising a first image showing a visually enhanced attribute of the subject, wherein the first image is generated from the data for the first time series of fluorescence images, and a second image showing the visually enhanced attribute of the subject, wherein the second image is generated from the data for the second time series of fluorescence images.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes receiving a time series of fluorescence images of the tissue of the subject wherein the images define a plurality of calculation regions, generating a plurality of time-intensity curves for the plurality of calculation regions, creating a set of parameter values for each calculation region, generating a total rank value for each calculation region by comparing the sets of parameter values, and converting the total rank value into a ranking map image. Also disclosed are methods and systems for characterizing a wound in tissue by generating a wound index value.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes receiving a time series of fluorescence images of the tissue of the subject wherein the images define a plurality of calculation regions, generating a plurality of time-intensity curves for the plurality of calculation regions, creating a set of parameter values for each calculation region, generating a total rank value for each calculation region by comparing the sets of parameter values, and converting the total rank value into a ranking map image. Also disclosed are methods and systems for characterizing a wound in tissue by generating a wound index value.

Abstract: Methods and systems for alignment of a subject for medical imaging are disclosed, and involve providing a reference image of an anatomical region of the subject, the anatomical region comprising a target tissue, processing the reference image to generate an alignment reference image, displaying the alignment reference image concurrently with real-time video of the anatomical region, and aligning the real-time video with the alignment reference image to overlay the real-time video with the alignment reference image. Following such alignment, the subject may be imaged using, for example, fluorescence imaging, wherein the fluorescence imaging may be performed by an image acquisition assembly aligned in accordance with the alignment.

Abstract: Systems and methods for storing data for a first time series of fluorescence images of the subject acquired during a first imaging session, storing data for a second time series of fluorescence images of the subject acquired during a second imaging session, receiving a request to view attributes of the subject, and in response to receiving the request, displaying a user interface on the display, the user interface comprising a first image showing a visually enhanced attribute of the subject, wherein the first image is generated from the data for the first time series of fluorescence images, and a second image showing the visually enhanced attribute of the subject, wherein the second image is generated from the data for the second time series of fluorescence images.

Abstract: Methods and systems for characterizing tissue of a subject are disclosed. The method includes receiving a time series of fluorescence images of the tissue of the subject wherein the images define a plurality of calculation regions, generating a plurality of time-intensity curves for the plurality of calculation regions, creating a set of parameter values for each calculation region, generating a total rank value for each calculation region by comparing the sets of parameter values, and converting the total rank value into a ranking map image. Also disclosed are methods and systems for characterizing a wound in tissue by generating a wound index value.