Abstract: The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retenate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one silica-based magnetic particle and having a pH ranging from 5 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the magnetic particle(s) to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with a low conductivity elution buffer to elute the purified nucleic acid from the magnetic particle. Kits comprising these buffers and magnetic particles are also disclosed herein.

Abstract: A system for clarifying a cell culture harvest solution, including target molecules and dynamic filter media. A filtration vessel includes a flexible liner and at least one filter having a surface on which the dynamic filter media accumulates into a cake. The cake and filter during filtration operation permit a filtrate including target molecules to pass therethrough while preventing unwanted solid materials from passing therethrough. A backflush source includes a backflush fluid and is fluidly connected to the filtration vessel via the at least one filter. The backflush source, during backflush operation, is adapted to supply backflush fluid back through the at least one filter for removing the cake formed on the filter. Related systems and methods are also disclosed.

Abstract: The invention provides a method and a system for the production of viruses or virus derived products. The system comprises a containment enclosure provided with a single entry means and a single exit means which are movable between a closed position and an open position. The containment enclosure comprises at least one production unit comprising at least one bioreactor for culturing the virus, said bioreactor is provided with at least one inlet and at least one outlet; at least one purification unit fluidly connected to the production unit and comprising at least one purification means having at least one inlet and at least one outlet. The system further comprises at least one inactivation and/or at least one gas decontamination unit. The containment enclosure comprises at least one process control device connected to the exit means. The movement of the exit means from the closed position to the open position is controllable by said process control device.

Abstract: A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.

Abstract: The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.

Abstract: Systems and methods for harvesting cells include a bioreactor with a structure for cell entrapment/adherence and growth, such as a fixed bed structure. A cell harvest mechanism is adapted to agitate the bioreactor, such as by vibrating or shaking the fixed bed, and to move a liquid level relative to the structure, such as by repeatedly flushing and filling the bioreactor. A cell detaching solution is provided to the bioreactor, such as an enzymatic cocktail for the detachment of cells without producing clumps or aggregates. The bioreactor may comprise a preculture vessel upstream of another bioreactor.

Abstract: An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Abstract: The invention provides a method and a kit for combined virus inactivation and capture/purification of a feed containing a protein of interest. The method comprises the steps of: contacting a feed comprising a protein of interest with negatively charged particles or media, conditioning the particles or media such that the pH is acidic thereby inactivating said virus, and eluting said protein of interest.

Abstract: The invention provides a method and a system for the production of viruses or virus derived products. The system comprises a containment enclosure provided with a single entry means and a single exit means which are movable between a closed position and an open position. The containment enclosure comprises at least one production unit comprising at least one bioreactor for culturing the virus, said bioreactor is provided with at least one inlet and at least one outlet; at least one purification unit fluidly connected to the production unit and comprising at least one purification means having at least one inlet and at least one outlet. The system further comprises at least one inactivation and/or at least one gas decontamination unit. The containment enclosure comprises at least one process control device connected to the exit means. The movement of the exit means from the closed position to the open position is controllable by said process control device.

Abstract: A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

Abstract: A management orchestrator for a content-centric network in a 6G network is described herein according to some aspects. For example, the management orchestrator can receive, from a client device, a request for a network function in the content-centric network executed by a plurality of network nodes in the 6G network. In response to receiving the request for the network function, the management orchestrator can identify a particular network node of the plurality of network nodes usable for executing the network function. The management orchestrator can then automatically deploy a named function via the particular network node. The named function can be used to execute the network function for the client device.

Abstract: A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

Abstract: An apparatus for culturing cells includes a bioreactor including a monolithic structured cell culture bed, which may be fabricated using additive manufacturing techniques, such as for example 3D printing. The bioreactor and monolithic structured cell culture bed may be formed as a unitary structure. The monolithic structured cell culture bed may include regions of varying porosity and/or surface treatments to achieve desired objectives. Related methods are also provided.

Abstract: The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

Abstract: A bioreactor includes a fixed bed for culturing cells and a sensor system for sensing a density of the cells in the fixed bed. The sensor may be selected from the group comprising: (a) a sensor for measuring impedance across at least a portion of the fixed bed; (b) a flowmeter for detecting a rate of flow of liquid associated with the fixed bed; (c) a sensor for measuring a pressure differential in a flow of liquid through the fixed bed; (d) a monitor, such as a light sensor or microscope, for detecting light from a light source for projecting light on or in the fixed bed, wherein the detected or measured characteristic is indicative of cell density in the fixed bed; (e) a chemical sensor within the fixed bed for detecting a chemical indicative of cell density in the fixed bed. Related sensor arrangements, systems, and methods are also disclosed.

Abstract: An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Abstract: An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Abstract: An apparatus for culturing cells with enhanced gas transfer and thermal regulation is provided. The apparatus includes a bioreactor with a cell culture bed, such as a fixed structured bed. A pump, such as an agitator, serves to pump liquid through the cell culture bed, and a container is provided for the agitator. A first conduit may be associated with the container, such as by being connected to it or adjacent an opening into it. The bioreactor may also include flow extenders to enhance the gas transfer to a liquid used as media to culture the cells, as well as optional thermoregulators. Related methods are also disclosed.