Patents by Inventor José Remacle

José Remacle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20070099204
    Abstract: Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
    Type: Application
    Filed: May 24, 2006
    Publication date: May 3, 2007
    Inventors: Isabelle Alexandre, Sylvain Margaine, Dieter Husar, Nathalie Zammatteo, Heinz Koehn, Jose Remacle
  • Patent number: 7205104
    Abstract: A method for identifying or quantifying an organism by a detecting its nucleotide sequence among at least 4 other homologous sequences comprising amplifying nucleic acids from the organism to generate target nucleotide sequences to be detected; contacting the target nucleotide sequences with single stranded capture nucleotide sequences bound by a single predetermined link to an insoluble solid support and discriminating the binding of a target nucleotide sequence specific of an organism with a signal resulting from a hybridization by complementary base pairing between the target nucleotide sequence and its corresponding capture nucleotide sequence is disclosed. The capture nucleotide sequence is bound to the insoluble solid support at a specific location on an array having a density of at least 4 different bound single stranded capture nucleotide sequences/cm2. The location of the signal on the array allows identification or quantification of the organism.
    Type: Grant
    Filed: March 23, 2001
    Date of Patent: April 17, 2007
    Assignee: Eppendorf Array Technologies SA (EAT)
    Inventors: José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville
  • Patent number: 7202026
    Abstract: The present invention is related to an identification and/or quantification method of a large number of biological organisms groups at different levels (family, genus, species) or part of those (possibly present in a biological sample) by a detection of their nucleotide sequence.
    Type: Grant
    Filed: January 23, 2002
    Date of Patent: April 10, 2007
    Assignee: Eppendorf Array Technologies SA (EAT)
    Inventors: José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville
  • Publication number: 20070072219
    Abstract: The present invention is related to a detection method and kit of homologous sequences differing by one base on a microarray.
    Type: Application
    Filed: September 12, 2006
    Publication date: March 29, 2007
    Inventors: Jose Remacle, Francoise De Longueville, Soumya Pastoret, Vincent Bertholet
  • Publication number: 20070037187
    Abstract: Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
    Type: Application
    Filed: May 17, 2006
    Publication date: February 15, 2007
    Inventors: Isabelle Alexandre, Sylvain Margaine, Dieter Husar, Nathalie Zammatteo, Heinz Koehn, Jose Remacle
  • Publication number: 20060281112
    Abstract: The invention relates to a method for the design and/or the preparation of a polynucleotide capture molecule for detecting an amplicon having one strand serving as target to be detected and/or quantified after hybridization on said capture molecule, comprising the steps of: (a) selecting a primer pair defining the amplicon; (b) selecting a specific sequence of 10 to 100 nucleotides within the amplicon, such that said specific sequence defines two non-complementary ends of the amplicon; (c) defining the capture molecule having a capture portion that is complementary to the specific sequence selected in step b), and a spacer portion comprising at least 20 nucleotides; and (d) identifying among the two non-complementary ends of the amplicon a spacer end and a non-spacer end, respectively, such that the spacer end is non-complementary to the spacer portion of the capture molecule, and said spacer end exceeds said non-spacer end by at least 50 bases.
    Type: Application
    Filed: May 17, 2006
    Publication date: December 14, 2006
    Inventors: Jose Remacle, Sandrine Hamels
  • Publication number: 20060134639
    Abstract: The present invention relates to a new method for determining the RNAi mediated transcriptional regulation of a cell by the determination of a pattern of at least 3 miRNA detected simultaneously and quantified in the same cell extract.
    Type: Application
    Filed: April 6, 2004
    Publication date: June 22, 2006
    Inventors: Christophe Huffel, Jose Remacle, Sven Bulow, Nathalie Zammatteo
  • Patent number: 7049064
    Abstract: The present invention is related to a method for making microarrays comprising the steps of: submitting the surface of a solid support to an oxidation of chemical groups present on said surface in order to allow the formation of aldehyde functions upon the surface of said solid support, covalently binding upon said aldehyde functions capture molecules designed for the detection, the identification, the quantification and/or the recovery of complementary target biological or chemical molecules of interest; said covalent binding resulting in an array comprising a density of at least 4 or more discrete regions/cm2 of solid support surface, each of said discrete surface regions being bound with a species of capture molecules.
    Type: Grant
    Filed: April 10, 2001
    Date of Patent: May 23, 2006
    Assignee: Eppendorf Array Technologies SA (EAT)
    Inventors: Laszlo Hevesi, Laurent Jeanmart, José Remacle
  • Publication number: 20060105354
    Abstract: The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at least 5 capture molecules (20) being immobilized in specifically localized areas (21) of said support; introducing said labeled target molecules solution (13) into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light (2) from a light source (1) on the surface of the micro-array; measuring the electromagnetic light emission (7) from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.
    Type: Application
    Filed: November 18, 2004
    Publication date: May 18, 2006
    Inventors: Jose Remacle, Isabelle Alexandre, Sylvain Margaine, Dieter Husar
  • Publication number: 20060099619
    Abstract: The present invention relates to a new method for the detection, identification and/or quantification of multiple gene-specific mRNA or stRNA, respectively, the inducers of RNAi. In particular the present invention relates to a method for detecting the presence or change in concentration of mRNA in a cell, which change may be induced by environmental conditions.
    Type: Application
    Filed: October 4, 2005
    Publication date: May 11, 2006
    Inventors: Jose Remacle, Sandrine Hamels, Francoise Longueville
  • Publication number: 20050272044
    Abstract: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each of the organisms possibly present in said biological sample, wherein said nucleotide sequence is homologous with at least 4 other nucleotide sequences.
    Type: Application
    Filed: June 2, 2004
    Publication date: December 8, 2005
    Inventors: Jose Remacle, Sandrine Hamels, Sophie Burteau, Nathalie Zammatteo
  • Publication number: 20050260648
    Abstract: The present invention relates to a new method for determining the RNAi mediated transcriptional regulation of a cell by the determination of a pattern of at least 3 miRNA detected simultaneously and quantified in the same cell extract.
    Type: Application
    Filed: April 8, 2005
    Publication date: November 24, 2005
    Inventors: Christophe Huffel, Jose Remacle, Sven Bulow, Nathalie Zammatteo
  • Publication number: 20050250109
    Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).
    Type: Application
    Filed: May 4, 2004
    Publication date: November 10, 2005
    Inventors: Lars-Erik Peters, Jose Remacle, Konrad Beyreuther
  • Publication number: 20050250108
    Abstract: The present invention is related to a method for the construction of micro-arrays of polynucleotide sets (3) on a surface (5) of a solid support to be used for the detection and/or the quantification of at least four different target polynucleotides or nucleotide sequences (4), possibly present in a biological sample or test solution, said method comprising the steps of fixing nucleotide line sequences (1) upon a surface (5) of the solid support, said nucleotide lines (1) being at least 20 nucleotides long and having a random nucleotide sequence, deposing in at least 4 specific locations (6) on the surface (5) of the comprising said fixed nucleotide line sequences (1), solid support, nucleotide hooks (2) having a sequence specific for one of said at least four different target polynucleotides sequences (4) to be detected and/or quantified and covalently linking at said specific surface location (6) the nucleotide hooks (2) to the nucleotide lines sequence (1) in order to form polynucleotide sets (3), specific
    Type: Application
    Filed: May 4, 2004
    Publication date: November 10, 2005
    Inventors: Jose Remacle, Konrad Beyreuther
  • Publication number: 20050227373
    Abstract: The present invention provides a method for capacitive detection of the presence of target sample on a substrate, which comprises the steps of: binding a target sample to selective binding sites on the substrate, the target sample being directly or indirectly labeled with conductive labels, and sensing the presence of the bound conductive labels to a binding site to thereby determine the presence of the target sample. The sensing step is carried out by a capacitive detection of the presence of the conductive labels. The present invention also provides a capacitive sensor device for determining the presence of a target sample. Conductive labels are directly or indirectly couplable to the target sample.
    Type: Application
    Filed: June 24, 2003
    Publication date: October 13, 2005
    Inventors: Denis Flandre, Luis Moreno, Remi Pampin, David Bourgeois, Jose Remacle, Pierre-Emmannuel Lobert
  • Publication number: 20050208518
    Abstract: The invention provides a tool and a method for the easy interpretation of the changes occurring in a cell, being a three dimensional complex and control system when transfected by RNAi. The method is based on the analysis of a limited number of data obtained by quantifying the intensity of the signals present on spots distributed in a two dimensional surface. The invention allows to observe the effects of the presence of a particular RNAI on the cells main vital cellular functions together with the possible side or deleterious effects resulting from the transfection process or to the presence of the RNAi in a cell.
    Type: Application
    Filed: November 12, 2004
    Publication date: September 22, 2005
    Inventors: Francoise Longueville, Jose Remacle
  • Publication number: 20050147978
    Abstract: The invention relates to a method for determining, whether a cell of interest is resistant to an active substance to which it is exposed, e.g. during cancer chemotherapy. The method comprises the quantitative analysis of the expression of at least 5 ATP binding cassette (ABC) transporter genes within a sample, treated in the same manner using a DNA micro-array.
    Type: Application
    Filed: December 30, 2003
    Publication date: July 7, 2005
    Inventors: Jose Remacle, Thomas Efferth, Jean-Pierre Gillet
  • Publication number: 20050112687
    Abstract: A method for stabilizing proteins immobilized on the surface of a solid support, or array, by incorporating polyols into the solutions to be dispensed. The polyols increase the time-dependent and thermal stability of all of the spotted proteins on the same support, thus conserving the micro-array for quantitative multiparametric analysis after a long storage period.
    Type: Application
    Filed: November 25, 2003
    Publication date: May 26, 2005
    Inventors: Jose Remacle, Gaetan Michel
  • Publication number: 20050059019
    Abstract: The present invention relates to a novel method for determining the biological effect of a siRNA species in a cell. In particular, the present invention provides a method, wherein a support is provided, containing on predetermined locations thereof nucleic acids, representing or giving rise to a siRNA species. The support is covered by cells, into which the siRNA are taken up and exert their effect. Then the biological effect of the siRNA in the cell may be determined.
    Type: Application
    Filed: September 11, 2003
    Publication date: March 17, 2005
    Inventors: Sven Bulow, Nathalie Zammatteo, Jose Remacle
  • Publication number: 20050053946
    Abstract: The invention pertains to a novel method for analyzing activation pathways controlled by neurotransmitters and to a micro-array for use in this method. In particular, the present method relates to the use of such a micro-array as a tool for investigation of several activation pathways in the rat brain, said activation pathways being under the control of amine receptors.
    Type: Application
    Filed: September 5, 2003
    Publication date: March 10, 2005
    Inventors: Jose Remacle, Francoise du Longueville