Abstract: Provided herein are systems and methods for electrically stimulating cultured cells, including cardiomyocytes. In particular, provided herein are systems and methods employing reusable electrode arrays with multiwell culture devices that provide electrical stimulation to cells that are cultured in individual wells of the devices. The technologies described may be used to stimulate cells for phenotype analysis.

Abstract: Provided herein are systems and methods for cardiomyocyte pacing of cultured cells, including cardiomyoctyes. In particular, provided herein are systems and methods employing electrode arrays with multiwell culture devices that provide electrical stimulation to cells cultured in individual wells of the devices.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived cardiomyocytes. In some embodiments, methods of generating cardiomyocytes from stem cells (e.g., induced pluripotent stem cells (iPS cells or IPSCs) and embryonic stem cells) are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Abstract: The present invention relates to compositions and methods for the prevention and treatment of atrial fibrillation. In particular, the present invention provides therapeutic agents for the treatment and prevention of persistent and permanent atrial fibrillation and prevention of progression of atrial fibrillation to permanent atrial fibrillation.

Abstract: The present invention relates to compositions and methods for the prevention and treatment of atrial fibrillation. In particular, the present invention provides therapeutic agents for the treatment and prevention of persistent and permanent atrial fibrillation and prevention of progression of atrial fibrillation to permanent atrial fibrillation.

Abstract: The invention relates to a method of high-resolution mapping of a heart, including: providing a heart mapping apparatus; contacting at least a portion of an intact heart tissue with a voltage-sensitive fluoroscopic dye to generate at least a portion of dyed heart tissue; inserting a first end of the heart mapping apparatus into an intact heart; illuminating the portion of dyed heart tissue with a first range of wavelengths of electromagnetic radiation from the first end of the heart mapping apparatus; collecting a second range of wavelengths of electromagnetic radiation from the portion of dyed heart tissue; and transforming the second range of wavelengths of electromagnetic radiation to at least about 100 points of information, wherein said 100 points of information yields a map of at least one anatomical feature and at least one electrical potential.

Abstract: A method and computer program product comprising an algorithm adapted to execute a method of identifying the spatial coordinates of a sustaining source of fibrillatory activity in a heart by computing a set of point-dependent dominant frequencies and a set of point-dependent regularity indices for a set of products of point-dependent unipolar discrete power spectra and point-dependent bipolar discrete power spectra, derived by spectral analyses of corresponding unipolar and bipolar cardiac depolarization signals simultaneously acquired from a set of points of the heart. A maximum dominant frequency is selected whose associated coordinates identify the point of the sustaining source of fibrillatory activity. The magnitude of the regularity index is interpreted to verify the identification of the spatial coordinates of the sustaining source of fibrillatory activity. When indicated, surgical intervention is directed to the spatial coordinates of the sustaining source of fibrillatory activity.

Abstract: A method and computer program product comprising an algorithm adapted to execute a method of identifying the spatial coordinates of a sustaining source of fibrillatory activity in a heart by computing a set of point-dependent dominant frequencies and a set of point-dependent regularity indices for a set of products of point-dependent unipolar discrete power spectra and point-dependent bipolar discrete power spectra, derived by spectral analyses of corresponding unipolar and bipolar cardiac depolarization signals simultaneously acquired from a set of points of the heart. A maximum dominant frequency is selected whose associated coordinates identify the point of the sustaining source of fibrillatory activity. The magnitude of the regularity index is interpreted to verify the identification of the spatial coordinates of the sustaining source of fibrillatory activity. When indicated, surgical intervention is directed to the spatial coordinates of the sustaining source of fibrillatory activity.

Abstract: Method and apparatus are provided for cardiac defibrillation using a waveform with a dominant frequency near that of a fibrillating heart. The defibrillator includes electrical generating means for producing a waveform having a dominant frequency within a range of 2 Hz-20 Hz. The waveform provides low level electrical shock to a fibrillating heart, thereby causing defibrillation. The waveform, which can comprise a Rossler-type chaotic waveform or a sinusoidal waveform, preferably has a dominant frequency near the frequency of fibrillation of the fibrillating heart.