Abstract: An apparatus and method for aligning the optical elements of a microscope are disclosed wherein a fixture is provided for locating and mounting a plurality of mirrors for reflecting an object image along an optical path to an adjacent eyepiece connected to the fixture by an eyepiece holder. The eyepiece holder includes an outer sleeve attachable to the fixture, an inner sleeve transversely movable within the outer sleeve for slidably receiving the eyepiece, and positioning means comprising a plurality of angularly spaced screws extending through the outer sleeve for engaging the inner sleeve, whereby the eyepiece may be aligned on the optical path by selectively adjusting the screws.