Abstract: Artificial dermis (1) obtained from plasma with platelets (2) and human fibroblasts. The plasma with platelets (2) is obtained from the fractionating of total blood (4) from the patient (8) by light centrifugation, and the human fibroblasts (3) from a skin biopsy, (5). Clotting is obtained by adding calcium. This artificial dermis (1) permits rapid growth of the keratinocytes (6) seeded on its surface to build an artificial skin (7) which can easily be transplanted. Large areas of artificial dermis (1) are obtained from small amounts of starting materials and can be enriched with cytokines and platelet growth factors, to strengthen proliferation of the cells seeded. The artificial skin (7) obtained can be used to treat major burn treatments, chronic skin ulcers, etc., or be used, by employing genetically altered cells, as a vehicle for gene therapy.

Abstract: Artificial dermis (1) obtained from plasma with platelets (2) and human fibroblasts. The plasma with platelets (2) is obtained from the fractionating of total blood (4) from the patient (8) by light centrifugation, and the human fibroblasts (3) from a skin biopsy, (5). Clotting is obtained by adding calcium. This artificial dermis (1) permits rapid growth of the keratinocytes (6) seeded on its surface to build an artificial skin (7) which can easily be transplanted. Large areas of artificial dermis (1) are obtained from small amounts of starting materials and can be enriched with cytokines and platelet growth factors, to strengthen proliferation of the cells seeded. The artificial skin (7) obtained can be used to treat major burn treatments, chronic skin ulcers, etc., or be used, by employing genetically altered cells, as a vehicle for gene therapy.

Abstract: The present invention belongs to the field of tissue engineering and specifically relates to a method for obtaining three-dimensional structures for tissue engineering and to the structures obtained by said method. The invention also relates to an ex vivo method for regenerating a tissue using the three-dimensional structures of the invention and to the use of the structures thus treated for their transplanting to the area to be regenerated of the patient.

Abstract: Artificial dermis (1) obtained from plasma with platelets (2) and human fibroblasts. The plasma with platelets (2) is obtained from the fractionating of total blood (4) from the patient (8) by light centrifugation, and the human fibroblasts (3) from a skin biopsy (5). Clotting is obtained by adding calcium. This artificial dermis (1) provides for the rapid growth of the keratinocytes (6) seeded on its surface to build an artificial skin (7) which can easily be transplanted. Large areas of artificial dermis (1) are obtained from a small skin biopsy (5) and minimal quantities of plasma with platelets (2), which being enriched with cytokines and platelet growth factors, strengthens the proliferation of the cells seeded, both inside and on the surface. The artificial skin (7) obtained can be used to treat major burn treatments, chronic skin ulcers, etc., or be used, by employing genetically altered cells, as a vehicle for gene therapy.

Abstract: Artificial dermis (1) obtained from plasma with platelets (2) and human fibroblasts. The plasma with platelets (2) is obtained from the fractionating of total blood (4) from the patient (8) by light centrifugation, and the human fibroblasts (3) from a skin biopsy (5). Clotting is obtained by adding calcium.

Abstract: A vertebrate, non-human transgenic animal whose cells, both somatic and germinal, contain a recombinant genetic construction formed of a marker gene and a regulator region capable of being induced in the epidermis of said transgenic animal in response to hyperproliferative stimuli, the genetic construction of which has been introduced into the animal or into an antecessor of the animal in an embryonic stage. The genetic construction is induced also in the bulb of the hair in the phase of active growth or anagen. The said genetic construction consists of a marker gene characterized by the fact that it is easily detectable, under the control of the regulator region of the gene of the K6 keratin, this protein being characterized by its ability to be induced in the suprabasal layers of the epidermis in response to hyperproliferative stimuli both endogenous (tumors, cicatrization of wounds, diseases which, such as psoriasis, produce hyperproliferation, etc.