Abstract: Aspects of the present disclosure include methods for integrating data quality assessment with data acquisition with a flow cytometer. Methods according to certain embodiments include detecting light with a light detection system from a sample irradiated by a light source in a flow stream, generating event data signals in response to events detected with the light detection system, binning the generated event data signals in a plurality of binning windows having overlapping data bins and assessing the plurality of binning windows for outlier events in the generated data signals. Systems having a processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

Abstract: Computer technology is disclosed that applies innovative data processing and visualization techniques to large multi-parameter data sets such as cellular gene expression data to find new relationships such as relationships between cells and genes and create new associative data structures within the data sets that represent these relationships. For example, scatterplots of gene expression data can be iteratively pivoted between a cell view and a gene view to find cell populations and gene sets of interest to a user.