Patents by Inventor Joseph A. Sorge

Joseph A. Sorge has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Humanized polynucleotide sequence encoding renilla mulleri green fluorescent protein
    Publication number: 20040146972
    Abstract: The present invention provides a polynucleotide encoding a green fluorescent protein from Renilla mulleri comprising a humanized sequence which permits enhanced expression of the encoded polypeptide in mammalian cells.
    Type: Application
    Filed: August 29, 2003
    Publication date: July 29, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Peter Edward Vaillancourt
  • Humanized polynucleotide sequence encoding renilla mulleri green fluorescent protein
    Publication number: 20040132180
    Abstract: The present invention provides a polynucleotide encoding a green fluorescent protein from Renilla mulleri comprising a humanized sequence which permits enhanced expression of the encoded polypeptide in mammalian cells.
    Type: Application
    Filed: August 29, 2003
    Publication date: July 8, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Peter Edward Vaillancourt
  • Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
    Publication number: 20040096956
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Application
    Filed: October 20, 2003
    Publication date: May 20, 2004
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • DNA polymerases with reduced base analog detection activity
    Publication number: 20040091873
    Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
    Type: Application
    Filed: November 18, 2002
    Publication date: May 13, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Holly Hogrefe, Madhushree Ghosh
  • DNA polymerases with reduced base analog detection activity
    Publication number: 20040086890
    Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
    Type: Application
    Filed: April 7, 2003
    Publication date: May 6, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Holly H. Hogrefe, Madhushree Ghosh
  • DNA polymerases with reduced base analog detection activity
    Publication number: 20040081965
    Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal dna polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
    Type: Application
    Filed: October 25, 2002
    Publication date: April 29, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Holly H. Hogrefe, Madhushree Ghosh
  • Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
    Patent number: 6696277
    Abstract: A method for generating a discrete set of DNA segments characteristic of a sample of a single-stranded RNA is provided.
    Type: Grant
    Filed: March 1, 1995
    Date of Patent: February 24, 2004
    Assignees: Stratagene, California Institute of Biological Research
    Inventors: Michael McClelland, John T. Welsh, Joseph A. Sorge
  • Methods of enriching for and identifying polymorphisms
    Publication number: 20040018512
    Abstract: The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample.
    Type: Application
    Filed: March 18, 2003
    Publication date: January 29, 2004
    Inventors: Joseph A. Sorge, Ronald W. Davis
  • Method for producing polymers having a preselected activity
    Patent number: 6680192
    Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: January 20, 2004
    Assignees: Scripps Research Institute, Medical Research Council, Stratagene
    Inventors: Richard A. Lerner, Joseph A. Sorge
  • Compositions and methods utilizing DNA polymerases
    Publication number: 20040009486
    Abstract: The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
    Type: Application
    Filed: August 19, 2002
    Publication date: January 15, 2004
    Inventors: Joseph A. Sorge, Connie Jo Hansen, Holly Hogrefe
  • Endonuclease VII cloning method
    Publication number: 20040002155
    Abstract: The subject invention provides for a strain of host cells that contains a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
    Type: Application
    Filed: June 26, 2002
    Publication date: January 1, 2004
    Applicant: Stratagene
    Inventors: Alan L. Greener, Lisa Joy Hexdall, Carsten-Peter Carstens, Joseph A. Sorge
  • Selective cloning of homoduplex nucleic acids
    Publication number: 20040002156
    Abstract: The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
    Type: Application
    Filed: September 19, 2002
    Publication date: January 1, 2004
    Applicant: Stratagene
    Inventors: Alan L. Greener, Lisa Joy Hexdall, Carsten-Peter Carstens, Joseph A. Sorge
  • DNA polymerase mutants with reverse transcriptase activity
    Publication number: 20030228616
    Abstract: The present invention relates to compositions and kits comprising a mutant DNA polymerase with increased reverse transcriptase activity. The invention also relates to methods for using the subject compositions and kits.
    Type: Application
    Filed: May 12, 2003
    Publication date: December 11, 2003
    Applicant: Stratagene
    Inventors: Bahram Arezi, Holly Hogrefe, Joseph A. Sorge, Connie Jo Hansen
  • Humanized polynucleotide sequence encoding Renilla mulleri green fluorescent protein
    Patent number: 6645761
    Abstract: The present invention provides a polynucleotide encoding a green fluorescent protein from Renilla mulleri comprising a humanized sequence which permits enhanced expression of the encoded polypeptide in mammalian cells.
    Type: Grant
    Filed: April 19, 2001
    Date of Patent: November 11, 2003
    Assignee: Stratagene
    Inventors: Joseph A. Sorge, Peter Edward Vaillancourt
  • Gene monitoring and gene identification using cDNA arrays
    Publication number: 20030207312
    Abstract: cDNA microarrays are provided for increasing the accuracy and reliability of expression profiling techniques and for identifying new genes. An array comprises a plurality of nucleic acid members, each member having a unique position and stably associated with a solid support. Each nucleic acid member comprises a noncoding sequence present at either the 3′-end or the 5′-end of an RNA transcript (e.g., such as an untranslated region or UTR). In one embodiment, each nucleic acid member is less than 1000 nucleotides. In another embodiment, each nucleic acid member is less than 600 nucleotides. In a further embodiment of the invention, each nucleic acid member comprises substantially noncoding sequences.
    Type: Application
    Filed: May 29, 2003
    Publication date: November 6, 2003
    Applicant: Stratagene
    Inventor: Joseph A. Sorge
  • Methods and kits to enrich for desired nucleic acid sequences
    Patent number: 6641998
    Abstract: The invention provides a method and related kits and reagents for producing, purifying, isolating, or enriching desired nucleic acids from a library. The desired nucleic acids are produced from polymerase enzyme extension products, generated from specific primers or sets of primers, in a form that is immediately replicable in a host cell. The invention can be practiced in solution phase, thus eliminating the need for solid phase filter hybridizations, column hybridizations, or gel electrophoresis purification when enriching for or isolating a target sequence or vector from one or more libraries.
    Type: Grant
    Filed: April 9, 1999
    Date of Patent: November 4, 2003
    Assignee: Stratagene
    Inventor: Joseph A. Sorge
  • Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
    Patent number: 6635424
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Grant
    Filed: March 2, 2001
    Date of Patent: October 21, 2003
    Assignee: Stratagene
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Vector for gene expression in prokaryotic and eukaryotic systems
    Patent number: 6627436
    Abstract: The invention concerns an expression vector that permits expression of genes and fragments thereof in both prokaryotic and mammalian systems. The invention also pertains to derivatives of such vector that contain one or more prokaryotic or eukaryotic (especially mammalian) genes or gene fragments. The invention further pertains to prokaryotic or mammalian cells containing such an expression vector or derivative.
    Type: Grant
    Filed: October 31, 1997
    Date of Patent: September 30, 2003
    Assignee: Stratagene
    Inventors: Joseph A. Sorge, Kerstien A. Padgett
  • High fidelity DNA polymerase compositions and uses therefor
    Publication number: 20030180741
    Abstract: The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3′-5′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
    Type: Application
    Filed: July 30, 2002
    Publication date: September 25, 2003
    Inventors: Holly Hogrefe, Michael Borns, Joseph Sorge
  • Compositions and methods comprising control nucleic acid
    Publication number: 20030175740
    Abstract: The present invention relates, in part, to control nucleic acid molecules having no significant sequence homology to any known nucleic acid, and predefined G/C-content. The present invention further relates to method of using control nucleic acid molecules to validate microarray analyses, compositions comprising control nucleic acid molecules, and kits comprising control nucleic acid molecules.
    Type: Application
    Filed: August 16, 2002
    Publication date: September 18, 2003
    Inventors: Rebecca Lynn Mullinax, Alexey Novoradovsky, Joseph A. Sorge