Abstract: The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer.

Abstract: The invention provides nucleic acid monomers with a 2?-modification that are useful for the incorporation of dyes or blocking groups. The monomers can be incorporated on the 3?-end of a dual labeled probe to inhibit PCR polymerase extension during PCR. The polymerase is inhibited from extending the probe at the 3?-hydroxyl group when the monomer is present; there is no need to add a chemical moiety to the 3?-hydroxyl or remove the 3?-hydroxyl. The monomers can also be incorporated internally or at the 5?-end of the oligonucleotide. A detectable label, such as a fluorescent or quenching dye, can be incorporated on the 2?-position of such monomers.

Abstract: The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.

Abstract: A method for preparing laminating materials and a composite prepared therefrom, the method including providing at least one layer of interleaf, extruding a thermoplastic adhesive with a die, forming a composite by applying the at least one layer of interleaf to the adhesive before cooling the adhesive, and pressing the composite with rolls to adhere the at least one layer of interleaf to the adhesive.

Abstract: The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer.

Abstract: Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition.

Abstract: A method for preparing laminating materials and a composite prepared therefrom, the method including providing at least one layer of interleaf, extruding a thermoplastic adhesive with a die, forming a composite by applying the at least one layer of interleaf to the adhesive before cooling the adhesive, and pressing the composite with rolls to adhere the at least one layer of interleaf to the adhesive.

Abstract: The use of oligodeoxynucleotides modified at the 3′-terminal internucleotide link as therapeutic agents by a method of hybridizing the modified oligonucleotide to a complementary sequence within a targeted mRNA and cleaving the mRNA within the RNA-DNA helix by the enzyme RNaseH to block the expression of the corresponding gene.

Abstract: The use of oligodeoxynucleotides modified at the 3'-terminal internucleotide link as therapeutic agents by a method of hybridizing the modified oligonucleotide to a complementary sequence within a targeted mRNA and cleaving the mRNA within the RNA-DNA helix by the enzyme RNaseH to block the expression of the corresponding gene.

Abstract: The use of oligodeoxynucleotides modified at the 3'-terminal internucleotide link as therapeutic agents by a method of hybridizing the modified oligonucleotide to a complementary sequence within a targeted mRNA and cleaving the mRNA within the RNA-DNA helix by the enzyme RNaseH to block the expression of the corresponding gene.

Abstract: A method of detection of nucleic acid (DNA or RNA) target sequence in which such a sequence serves as a cofactor for a catalytic reaction in which a complementary, labeled nucleic acid probe is cleaved such that the target sequence is released intact and can repeatedly recycle through the reaction pathway, thereby providing signal amplification.

Abstract: A high yield method of production of a cross-linked hemoglobin derivative suitable for use as a blood substitute and plasma expander which is cross-linked between Lys 99 Alpha.sub.1 and Lys 99 Alpha.sub.2 in high percentage by adding the cross-linker in the presence of an added polyanion which blocks competing reactions at other sites of the protein.

Abstract: Blood substitute and blood plasma expanders comprising alpha-alpha cross-linked stroma-free hemoglobin, which is substantially free of hemoglobin derivatives modified at other sites. The hemoglobin composition is intramolecularly cross-linked between Lys 99 Alpha.sub.1 and Lys 99 Alpha.sub.2.

Abstract: A high yield method of production of a cross-linked hemoglobin derivative suitable for use as a blood substitute and plasma expander which is cross-linked between Lys 99 Alpha.sub.1 and Lys 99 Alpha.sub.2 in high percentage by adding the cross-linker in the presence of an added polyanion which blocks competing reactions at other sites of the protein.