Patents by Inventor Joseph Barbieri

Joseph Barbieri has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20070059326
    Abstract: A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparing E. coli transformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.
    Type: Application
    Filed: November 30, 2005
    Publication date: March 15, 2007
    Inventors: Michael Baldwin, Marite Bradshaw, William Tepp, Eric Johnson, Joseph Barbieri, Christina Pier
  • Publication number: 20060258847
    Abstract: The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.
    Type: Application
    Filed: April 14, 2006
    Publication date: November 16, 2006
    Inventors: Eric Johnson, Marite Bradshaw, Michael Baldwin, Joseph Barbieri