Abstract: The disclosure provides a novel method for treating genetic disorders where a peptide sequence targets proteins produced via gene therapy into exosomes. These protein-loaded exosomes can enter into non-transduced cells and correct pathology. Also, gene therapy compositions, protein replacement therapy composition, pharmaceutical compositions, methods of treatment, and uses of the gene therapy compositions and the recombinant proteins are also disclosed. The method can also be used to improve in vitro recombinant protein yield.

Abstract: The present invention provides engineered novel variants of human immunodeficiency virus reverse transcriptase (HIV-RT) capable of being expressed in large quantity and that with polymerase and RNase H activity in a form that facilitates crystallization and high resolution structure resolution following X-ray diffraction. The present invention facilitates high resolution determination of RT in complexes with RT drugs and RT inhibitors, and provides methods for systematic generation of variants and for structure based identification and design of novel RT inhibitors.

Abstract: A printer receives commands from a host device in association with a print request, determines if the printer's resources permit processing of the commands received from the host when in a non-discard mode, enters the printer into a discard mode when resources associated with the printer are not available to permit processing the commands and, unless a command is protocol control command, discards commands to maintain communication between the printer and the host. The printer exits discard mode when a command received from the host is a protocol control command requesting that the printer exit discard mode. The host buffers commands associated with the print request, transmits commands to the printer for printing, receives indication that the printer is entering into a discard mode and resends the commands buffered on the host to the printer when the printer indicates it has exited discard mode.