Abstract: Disclosed are methods, systems, and non-transitory computer readable media having stored thereon executable instructions that when executed by the processor of a computer control the computer to perform therapeutic ultrasound, such for the treatment of Ischemic reperfusion injury (IRI). The methods, systems, and non-transitory computer readable media can involve steps comprising: imaging a target tissue region within said subject, such as wherein the target tissue region comprises spleen tissue; identifying a volume region of interest (ROI) in said target tissue; and applying ultrasonic energy to the ROI, wherein applying the ultrasonic energy comprises emitting a sequence of ultrasonic pulses from an ultrasound transducer, the sequence of ultrasound pulses having predetermined frequency, mechanical index, pulse lengths, and pulse spacings, while performing a volumetric sweep through the ROI systematically for a selected total time duration, such as between about 3 minutes and about 15 minutes.