Abstract: The present disclosure relates to methods of synthesizing moderate length RNAs by splint-mediated ligation of RNA fragments.

Abstract: A new, unique, lightweight and compact dog waste-collection device and bag disposal system is disclosed for the sanitary and convenient capture and disposal of dog waste comprised of a telescoping plastic handle and a collection bag rim which can be easily transformed from the closed “carry” position 120 degrees to the open “working” position. A new, specific, disposable, 100% biodegradable and 100% compostable waste-collection bag which is quickly and easily affixed to the device by sliding a hole in the bag down and around the device's handle, pushing the bag through the inside of the collection bag rim allowing the top 2? or so of the bag to fold over the collection bag rim. The device is positioned behind an eliminating dog and catches the waste before it hits the ground. The bag is removed with a simple downward pull and disposed of in an environmentally-responsible manner.