Abstract: Techniques are disclosed herein for iceberg order processing by an exchange system that includes storing a first representation of an iceberg order requested by a user in an order book and automatically causing a second representation to be stored for the iceberg order in the order book after the displayed quantity associated with the first representation is depleted/zeroed or otherwise below a predetermined threshold following execution of an associated trade. The second representation of an iceberg order can be referred to as a reclip order, and N number of such reclip orders can be stored/accepted in this manner by the exchange system until a hidden/reserve quantity (also referred to herein as a remaining iceberg quantity) is zero or the user who originated the iceberg order submits a cancel request.

Abstract: Provided are compositions and methods for transposon-CRISPR-based DNA editing. The compositions and methods include a modified TnsA protein that is altered from its original sequence found in Aeromonas salmonicida, and provides improved transposition frequency, and is functional in a heterologous system. The TnsA protein is used in a system with transposon proteins TnsB, TnsC, TniQ, Cas proteins Cas8f, Cas5f, Cas7f, Cas6f, a transposable DNA cargo sequence that is flanked by left and right transposon sequences, and at least one guide RNA that contains at least one spacer targeted to a target DNA sequence in the chromosome or the extrachromosomal element.

Abstract: Provided are gain-of-activity mutations in components of a type I-F3 Tn7-CRISPR-Cas system. The mutations allow use of systems that contain proteins with the mutations to function with both typical and atypical guide RNAs, and permit altered protospacer adjacent motif (PAM) usage.

Abstract: Provided are compositions and methods for transposon-CRISPR-based DNA editing. The compositions and methods include a modified TnsA protein that is altered from its original sequence found in Aeromonas salmonicida, and provides improved transposition frequency, and is functional in a heterologous system. The TnsA protein is used in a system with transposon proteins TnsB, TnsC, TniQ, Cas proteins Cas8f, CasSf, Cas7f, Cas6f, a transposable DNA cargo sequence that is flanked by left and right transposon sequences, and at least one guide RNA that contains at least one spacer targeted to a target DNA sequence in the chromosome or the extrachromosomal element.

Abstract: Provided are type I-D CRISPR-associated transposon (CAST) systems. The systems can be used with modified guide RNAs that are self-processing, and can be adapted to include binding sites for non-CAST proteins or polynucleotides. The systems may exclude a Cas6 protein. Methods of using the CAST systems for modifying DNA in heterologous hosts are also included.

Abstract: Provided are compositions and methods for modifying DNA substrates. The compositions include modified I-F3 proteins for use in a CRISPR systems to modify a DNA substrate. The modified proteins include I-F3 TnsC, TniQ, TnsA, TnsB and fusion proteins containing TnsA and TnsB, Cas8, Cas5, Cas7, and Cas6 modified proteins. The CRISPR systems include a guide RNA. Protein modifications provide for a higher transposition frequency than unmodified I-F3 CRISPR systems.

Abstract: Provided are compositions, methods, systems, and kits, for use in CRISPR-based DNA editing. The compositions include RNA polynucleotides that include one or more atypical repeats, and can include truncated spacers. The RNA polynucleotides are used with proteins in systems that include CRISPR and transposon genes, or proteins encoded by the genes. The genes include transposon genes tnsA, tnsB, tnsC, and tniQ, and Cas genes cas8f, cas5f, cas7f, and cas6f. Use of the RNA polynucleotides as guide RNAs that include atypical repeats with the transposon and CRISPR proteins exhibit enhanced transposition, relative to guide RNAs that do not include atypical repeats. Enhanced transposition is demonstrated using representative IF-3b systems.

Abstract: Provided are compositions and methods for transposon-CRISPR-based DNA editing. The compositions and methods include a modified TnsA protein that is altered from its original sequence found in Aeromonas salmonicida, and provides improved transposition frequency, and is functional in a heterologous system. The TnsA protein is used in a system with transposon proteins TnsB, TnsC, TniQ, Cas proteins Cas8f, CasSf, Cas7f, Cas6f, a transposable DNA cargo sequence that is flanked by left and right transposon sequences, and at least one guide RNA that contains at least one spacer targeted to a target DNA sequence in the chromosome or the extrachromosomal element.

Abstract: A multilayer membrane separator which permits serial and tangential contact of a retentate with a plurality of membranes within the separator. The separator is formed from a plurality of contiguous separator units each consisting of two membrane layers, two retentate layers, a filtrate spacer layer and two adhesive layers. The adhesive layers, in cooperation with a liquid inlet to the separator and a liquid outlet from the separator effect the desired retentate flow and prevent mixing of retentate and filtrate.

Abstract: A multiwell filtration apparatus for the assay of microliter quantities is provided which prevents fluid loss by capillary action and gravity flow through a microporous membrane or ultrafilter. The filtration apparatus is particularly advantageous in assays requiring maintenance of fluid within the reaction wells for substantial time periods and in small sample volume assays in the range of 100 microliter volumes.