Patents by Inventor Joseph Edward Shultz

Joseph Edward Shultz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Capture purification processes for proteins expressed in a non-mammalian system
    Patent number: 8940878
    Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: January 27, 2015
    Assignee: Amgen Inc.
    Inventors: Joseph Edward Shultz, Roger Hart
  • CAPTURE PURIFICATION PROCESSES FOR PROTEINS EXPRESSED IN A NON-MAMMALIAN SYSTEM
    Publication number: 20100331526
    Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
    Type: Application
    Filed: June 24, 2010
    Publication date: December 30, 2010
    Applicant: AMGEN INC.
    Inventors: Joseph Edward Shultz, Roger Hart
  • REFOLDING PROTEINS USING A CHEMICALLY CONTROLLED REDOX STATE
    Publication number: 20100324269
    Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
    Type: Application
    Filed: June 21, 2010
    Publication date: December 23, 2010
    Applicant: AMGEN INC.
    Inventors: Joseph Edward Shultz, Roger Hart, Ronald Nixon Keener, III