Patents by Inventor Joseph Francis Shuga
Joseph Francis Shuga has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11970739Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.Type: GrantFiled: July 6, 2023Date of Patent: April 30, 2024Assignee: 10x Genomics, Inc.Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
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Patent number: 11884964Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: July 11, 2022Date of Patent: January 30, 2024Assignee: 10X GENOMICS, INC.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
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Publication number: 20240011090Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.Type: ApplicationFiled: July 6, 2023Publication date: January 11, 2024Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
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Patent number: 11739381Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.Type: GrantFiled: July 18, 2022Date of Patent: August 29, 2023Assignee: 10x Genomics, Inc.Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
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Publication number: 20220389504Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.Type: ApplicationFiled: July 18, 2022Publication date: December 8, 2022Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
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Publication number: 20220364150Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: July 11, 2022Publication date: November 17, 2022Applicant: 10x Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
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Patent number: 11441172Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: September 1, 2020Date of Patent: September 13, 2022Assignee: 10x Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
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Publication number: 20210115415Abstract: Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.Type: ApplicationFiled: December 7, 2020Publication date: April 22, 2021Applicant: 10X Genomics, Inc.Inventors: Josephine Lee, Samuel Marrs, Geoffrey McDermott, Francesca Meschi, Luz Montesclaros, Katherine Pfeiffer, Joseph Francis Shuga, Jessica Michele Terry, Solongo B. Ziraldo
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Publication number: 20200392564Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: September 1, 2020Publication date: December 17, 2020Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
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Patent number: 10837047Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: April 3, 2019Date of Patent: November 17, 2020Assignee: 10X Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
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Patent number: 10774369Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: April 3, 2019Date of Patent: September 15, 2020Assignee: 10X Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
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Publication number: 20190233878Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: April 3, 2019Publication date: August 1, 2019Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
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Publication number: 20180312822Abstract: Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.Type: ApplicationFiled: May 9, 2018Publication date: November 1, 2018Inventors: Josephine Lee, Samuel Marrs, Geoffrey McDermott, Francesca Meschi, Luz Montesclaros, Katherine Pfeiffer, Joseph Francis Shuga, Jessica Michele Terry, Solongo B. Ziraldo