Abstract: A solvent delivery system (10) has first and second pistons (92, 94) and cylinders (58, 68) connected to first and second cams (110, 112). The cams (110, 112) each have a ramped portion (150, 162) and an abrupt step (152, 164). The first cylinder (58) is connected to solenoid valves (44, 46, 48) to fill the first cylinder with solvent components during movement of cam follower (104) along ramped portion (150) of the first cam (110). Spring (140) urges the first piston (92) forward into first cylinder (58). Movement of the cam follower (104) over the step (152) of the cam (110) moves the piston (92) forward abruptly to force the solvent components from cylinder (58) into second cylinder (68). Spring (178) urges piston (94) back in cylinder (68). The second cam (112) is reversed relative to first cam (110). Movement of cam follower (106) over abrupt step (164) of second cam (112) moves second piston (94) back abruptly in cylinder (68) to receive and mix the solvent components in the cylinder (68).

Abstract: Particle analyzing method and apparatus are provided for measuring the intensity of light scattered from a particle as a function of angle. Particles such as biological cells are contained in a stream for travel along a path which includes a scatter line along which the particles pass successively. A beam of collimated monochromatic radiation is directed onto the flow path, and detector means are provided having a radiation acceptance geometry whose projection intercepts the beam along the scatter line. The projected acceptance geometry includes an aperture image located adjacent the scatter line and having a dimension in the direction of travel of the particles which is substantially less than the length of the scatter line such that the angle of scatter radiation detectable by the detector means varies according to the position of the particle along the scatter line. The output from the detector provides a measure of the scatter light intensity versus angle characteristic of the particle.