Abstract: A fishing line spool holder for a tackle box having a tray therein, for facilitating the transfer of fishing line from the fishing line spool holder to a fishing reel, the spool holder including a spindle on which the fishing spool is rotatably mounted, a support for holding the spindle and means for engaging the support with the tackle box tray. One end of the spindle is adjustedly located at different distances from the tray for transferring fishing line from different size spools which are rotatedly mounted on these spindles between the outer end thereof and the tray.