Abstract: A tissue fragmenting device and methods of using the same are provided herein. A device for preparing a plurality of tissue fragments from a tissue sample includes a platform having a central opening, a shuttle slidably coupled to the platform and including a shuttle blade, a blade array mounted across the central opening of the platform having a plurality of blades each generally perpendicular to the shuttle blade, and a piston slidably coupled to the platform and slidably coupled to the shuttle along a predefined raceway and configured to urge the tissue sample into the blade array.

Abstract: The present invention provides improved methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for cryopreserving tumor tissues that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

Abstract: The present invention provides automated methods for expanding TILs and producing therapeutic populations of TILs, including novel tissue culture devices and automated methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs.

Abstract: A gas permeable cell culture device including a container body defining an interior volume, the container body having a semi-permeable membrane defining one end of the interior volume, a fitting defining an opposing end of the interior volume, and a cell transfer conduit. The cell transfer conduit having an open end positioned within the interior volume between the semi-permeable membrane and the fitting and a radial portion disposed within the interior volume and between the open end and opposing end of the container body.