Patents by Inventor Joseph Jollick
Joseph Jollick has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10088481Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: GrantFiled: January 21, 2015Date of Patent: October 2, 2018Assignee: Diagnostic Hybrids, Inc.Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, Jr., Ronald Lollar
-
Publication number: 20150192584Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: ApplicationFiled: January 21, 2015Publication date: July 9, 2015Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, JR., Ronald Lollar
-
Patent number: 8975016Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: GrantFiled: June 22, 2011Date of Patent: March 10, 2015Assignee: Diagnostic Hybrids, Inc.Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, Jr., Ronald Lollar
-
Publication number: 20120202189Abstract: A novel cytometer system, methods and algorithms which provide a rugged, affordable and easy-to-use device in remote locations. All cells in a biological sample are fluorescently labeled, with target cells also magnetically labeled. Non-magnetically labeled cells are imaged for viability. Labeled sample is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells for observation. A LED illuminates the cells and a CCO camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a cell count that can be related to the target cell concentration of the original sample.Type: ApplicationFiled: April 16, 2008Publication date: August 9, 2012Inventors: Mark Carle Connelly, Frank A.W. Coumans, Jimmy Page, Galla Chandra Rao, Joseph Jollick, JR.
-
Publication number: 20120003627Abstract: The present invention describes a device for performing a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The device also includes a compatible slide comprising sample wells. The device detects emitted fluorescence signal through a camera and optics assembly that is controlled by a user interface assembly.Type: ApplicationFiled: June 23, 2011Publication date: January 5, 2012Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, JR., Ronald H. Lollar, Brooke B. McCutchan
-
Publication number: 20110269117Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: ApplicationFiled: June 22, 2011Publication date: November 3, 2011Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, JR., Ronald Lollar
-
Patent number: 8003314Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: GrantFiled: April 16, 2009Date of Patent: August 23, 2011Assignee: Diagnostic Hybrids, Inc.Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, Jr., Ronald Lollar
-
Publication number: 20100261153Abstract: The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.Type: ApplicationFiled: April 16, 2009Publication date: October 14, 2010Inventors: David R. Scholl, James L. Brown, Joseph A. Jollick, JR., Ronald Lollar
-
Publication number: 20100173280Abstract: The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents.Type: ApplicationFiled: September 19, 2006Publication date: July 8, 2010Applicant: Diagnostic Hybrids, IncInventors: Yung T. Huang, Joseph A. Jollick, JR.
-
Patent number: 7435588Abstract: The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents.Type: GrantFiled: April 28, 2006Date of Patent: October 14, 2008Assignee: Diagnostic Hybrids, Inc.Inventors: Yung T. Huang, Joseph A. Jollick, Jr.
-
Patent number: 7341829Abstract: The present invention provides methods and compositions for the in situ growth, freezing and testing of cultured cells. In particular, the present invention provides methods and compositions for the long-term preservation of cells in ready-to-use formats for testing. In addition, the present invention provides rapid and easy to use means to diagnose viral and other infections. Furthermore, the present invention provides easy to use means to grow and store cells in situ for testing methods. Indeed, the present invention makes viral, chlamydial and other diagnostic methods accessible to small laboratories, including those without cell culture capabilities.Type: GrantFiled: September 9, 2002Date of Patent: March 11, 2008Assignee: Diagnostic Hybrids, Inc.Inventors: David R. Scholl, Francesco Saverio Ambesi-Impiombato, James L. Brown, Leonard D. Kohn, Joseph A. Jollick, Jr.
-
Publication number: 20070065939Abstract: The present invention generally relates to the field of diagnostic microbiology, and, more particularly to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents.Type: ApplicationFiled: April 28, 2006Publication date: March 22, 2007Applicants: University Hospitals of Cleveland, Diagnostic Hybrids, Inc.Inventors: Yung Huang, Joseph Jollick
-
Publication number: 20060094105Abstract: The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents.Type: ApplicationFiled: September 20, 2005Publication date: May 4, 2006Applicants: University Hospitals of Cleveland, Diagnostic Hybrids, Inc.Inventors: Yung Huang, Joseph Jollick
-
Publication number: 20050095582Abstract: The invention provides compositions and methods for detecting the presence of SARS-coronavirus, for screening anti-SARS coronavirus agents and vaccines, and for reducing infection with plus-strand RNA viruses such as SARS-coronavirus.Type: ApplicationFiled: November 3, 2003Publication date: May 5, 2005Applicants: Diagnostic Hybrids, Inc., Health Research IncorporatedInventors: Laura Gillim-Ross, Jill Taylor, David Scholl, David Wentworth, Joseph Jollick
-
Publication number: 20030113911Abstract: The present invention provides methods and compositions for the in situ growth, freezing and testing of cultured cells. In particular, the present invention provides methods and compositions for the long-term preservation of cells in ready-to-use formats for testing. In addition, the present invention provides rapid and easy to use means to diagnose viral and other infections. Furthermore, the present invention provides easy to use means to grow and store cells in situ for testing methods. Indeed, the present invention makes viral, chlamydial and other diagnostic methods accessible to small laboratories, including those without cell culture capabilities.Type: ApplicationFiled: September 9, 2002Publication date: June 19, 2003Inventors: David R. Scholl, Francesco Saverio Ambesi-Impiombato, James L. Brown, Leonard D. Kohn, Joseph A. Jollick
-
Patent number: 6472206Abstract: The present invention provides methods and compositions for the in situ growth, freezing and testing of cultured cells. In particular, the present invention provides methods and compositions for the long-term preservation of cells in ready-to-use formats for testing. In addition, the present invention provides rapid and easy to use means to diagnose viral and other infections. Furthermore, the present invention provides easy to use means to grow and store cells in situ for testing methods. Indeed, the present invention makes viral, chlamydial and other diagnostic methods accessible to small laboratories, including those without cell culture capabilities.Type: GrantFiled: April 28, 2000Date of Patent: October 29, 2002Assignees: Interthyr Corporation, Diagnostic Hybrids, Inc.Inventors: David R. Scholl, Francesco Saverio Ambesi-Impiombato, James L. Brown, Leonard D. Kohn, Joseph A. Jollick, Jr.