Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: The invention provides methods for manufacturing optimized CAR T cell therapies and uses thereof. Specifically, the invention provides parameters that can be measured, e.g., evaluated, to manufacture CAR T cell therapies with optimized properties. The invention further provides methods of use in connection with said optimized CAR T cells.

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: Cell culture devices comprise a frame comprising an open well disposed therein and a fluid inlet area in communication with the open well. The open well comprises a top opening, a bottom plate comprising a microcavity substrate, the bottom plate defining a major surface, and one or more sidewalls extending from the bottom plate to the top opening. The microcavity substrate comprises a plurality of microcavities, and each microcavity is configured such that cells cultured in the well form a spheroid. The cell culture device may be a reservoir open well microcavity plate used for spheroid cell culture.

Abstract: An anesthesia vaporizer system includes a vaporizer reservoir forming a sump chamber that contains liquid anesthetic agent and a fill chamber isolated from the sump chamber configured to receive liquid anesthetic agent from a refill container. A piston is in the sump chamber configured to maintain a constant force on the liquid anesthetic agent in the sump chamber, and a transfer valve is configured to permit the liquid anesthetic agent to flow from the fill chamber to the sump chamber. The vaporizer reservoir has an outlet port that delivers the liquid anesthetic agent from the sump chamber to a vaporizing system that vaporizes the liquid anesthetic agent for delivery to a patient.

Abstract: A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

Abstract: Vial assemblies comprise a tubular body and a cap, the cap including a first portion configured to abut a lip of an open end of the tubular body, a threaded portion configured to couple to threading on an internal surface of the tubular body, and a second portion protruding from the threaded portion and extending into a cavity of the tubular body. Methods for storing and removing frozen samples from such vial assemblies are also described.

Abstract: A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

Abstract: A cell culture system for culturing cells is provided. The cell culture system including a cell culture vessel with a cell culture chamber for culturing cells, an inlet through which liquid can flow for filling the cell culture vessel, and an outlet through which fluid can exit the cell culture vessel. The system also includes at least one fill sensor arranged to detect liquid within the cell culture vessel reaching a fill level at a predetermined position of the cell culture vessel during filling of the cell culture vessel, where the at least one fill sensor can generate a detection signal when liquid within the cell culture vessel reaches the fill level. Also included is an actuator to change an orientation of the cell culture vessel in response to the detection signal.

Abstract: Vial assemblies comprise a tubular body and a cap, the cap including a first portion configured to abut a lip of an open end of the tubular body, a threaded portion configured to couple to threading on an internal surface of the tubular body, and a second portion protruding from the threaded portion and extending into a cavity of the tubular body. Methods for storing and removing frozen samples from such vial assemblies are also described.

Abstract: A cell culture system is provided that includes a cell culture vessel having an interior cavity to house a cell culture substrate in a cell culture space, and at least one port for at least one of fluid inlet to the interior cavity and fluid outlet from the interior cavity. The system further includes a piston having a distal end disposed in the cell culture vessel above the cell culture space, the distal end of the piston being sealed with an airtight seal within the interior cavity. The system also includes a driver coupled to the piston to move the piston so as to increase and decrease a distance between the distal end and the cell culture space. The driver can pressurize the interior cavity via actuation of the piston to harvest cells from the cell culture space through the at least one port.

Abstract: Vial assemblies comprising a tubular body and a flexible bottom or flexible liner are described herein, wherein the flexible bottom or liner is configured to compress at least partially upon the application of force. Methods for storing and removing frozen samples from such vial assemblies are also described herein.

Abstract: A cell culture assembly includes a film defining a plurality of wells extending into the film, where each well of the plurality of wells defines a perimeter extending around the well, the plurality of wells defines an outer perimeter extending around the plurality of wells and extending between the perimeters of adjacent wells of the plurality of wells, and the outer perimeter extending around the plurality of wells defines a wave shape, and a sidewall member coupled to the film, where at least a portion of the sidewall member is oriented transverse to the film, the sidewall member including an engagement portion defining a wave shape that is complementary with the outer perimeter of the plurality of wells.

Abstract: A system for tracking anesthetic agent in a vaporizer reservoir can include a mass flow sensor configured to measure a flow rate entering or exiting a vaporizer chamber, a gas pressure sensor configured to measure a pressure of a mixed gas provided from the vaporizer chamber, and a processor to calculate a remaining agent time based on at least the gas flow rate, the pressure of the mixed gas, and an anesthetic concentration in the mixed gas. The processor can also provide the remaining agent time for display to a clinician.

Abstract: Various implementations of a pipette tip system are disclosed having a number of innovative features. In one implementation, a pipette tip tray includes a support plate and one or more sleeves extending downward from the bottom of the support plate. The sleeves can be configured to increase the stability of pipette tips stored in the tray. In another implementation, a pipette tip rack includes a rack base having one or more support walls extending underneath a pipette tip tray having downward extending sleeves. The thickness of the sidewalls of the sleeves can be reduced in the area of the support walls so that the support walls can reach the bottom of the support plate. In another implementation, a pipette tip rack includes a cover having a cushioning layer on the bottom of the cover that contacts the top of the pipette tips and stabilizes them during handling and transport.

Abstract: A system for tracking anesthetic agent in a vaporizer reservoir includes a mass flow sensor configured to measure a flow rate entering or exiting a vaporizer chamber, a gas pressure sensor configured to measure a pressure of a mixed gas provided from the vaporizer chamber, a gas temperature sensor configured to measure a temperature of the mixed gas provided from the vaporizer chamber, and an agent time module executable on a processor. The agent time module is configured to calculate a remaining agent time based on at least the gas flow rate, the pressure of the mixed gas, the temperature of the mixed gas, and an anesthetic concentration in the mixed gas. The remaining agent time is then provided for display on a display device.

Abstract: An anesthesia vaporizer system includes a vaporizer reservoir forming a sump chamber that contains liquid anesthetic agent and a fill chamber isolated from the sump chamber configured to receive liquid anesthetic agent from a refill container. A piston is in the sump chamber configured to maintain a constant force on the liquid anesthetic agent in the sump chamber, and a transfer valve is configured to permit the liquid anesthetic agent to flow from the fill chamber to the sump chamber. The vaporizer reservoir has an outlet port that delivers the liquid anesthetic agent from the sump chamber to a vaporizing system that vaporizes the liquid anesthetic agent for delivery to a patient.

Abstract: A flask assembly that includes: an Erlenmeyer flask; and a liner bag comprising a single opening the bag sized to fit within the flask and having a thickness from about 0.0254 mm to about 0.508 mm. The liner bag is configured for cell culturing and ease of insertion of the bag into the flask. In addition, the liner bag comprises a gas-permeable, polymeric material. Further, the liner bag can include a pocket adapted to receive a removable flask insertion element and/or at least one foldable seam configured to facilitate insertion of the bag into the flask. The liner bag can also include a vent having a gas permeability that is greater than the gas permeability of the gas-permeable, polymeric material employed in the balance of the bag.

Abstract: An anesthesia vaporizer system includes a vaporizer reservoir forming a sump chamber containing a liquid anesthetic agent, a piston that forms a first chamber on a first side of the piston. The vaporizer reservoir has a fill port configured to receive a refill container containing liquid anesthetic agent and to permit flow into the first chamber. A motor moves the piston between a first position and a second position, wherein the volume of the first chamber increases as the piston moves from the first position towards the second position. The liquid anesthetic agent is thereby drawn from the refill container into the first chamber. A transfer valve is configured to permit the liquid anesthetic agent to flow from the first chamber into the sump chamber. An outlet port delivers the liquid anesthetic agent from the sump chamber into a vaporizing system that vaporizes it for delivery to a patient.

Abstract: A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.