Abstract: A unique package for inoculants and a method for enabling the end user to introduce a pure culture of a microorganism into its appropriate growth substance, and later the microorganism enriched substance into a carrier material within a self-contained unit. The basic package separates a stabilized pure culture of the microorganism from the growth substance by a clamp which forms a physical partition. The microorganism is introduced to the substance when the partition is eliminated. After a time of growth to a maximum extent possible in the substance, the package is fractured to release the fresh inoculation at maximum microorganism vitality. Growth of the microorganism in the growth substance in-situ results in maximum numbers of the beneficial microorganism being available at the site of application.