Patents by Inventor Joseph S. Schoeniger
Joseph S. Schoeniger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240033362Abstract: A construct includes a core comprising an external surface and a plurality of pores, a cargo disposed in a pore of the plurality of pores, the cargo comprising a CRISPR Cas9 component or a nucleic acid sequence encoding a CRISPR Cas9 component; and a coating coupled to the core, wherein the coating comprises a cationic lipid, a pegylated lipid, a zwitterionic lipid, and a sterol. The coating may comprise a molar ratio of about 1 cationic lipid to 1 zwitterionic lipid to 0.9 sterol to 0.15 PEGylated lipid, wherein each molar ratio optionally varies by about plus or minus 10%. A method of treatment is also provided.Type: ApplicationFiled: July 27, 2022Publication date: February 1, 2024Inventors: Oscar Negrete, Annette Estelle LaBauve, Joseph S. Schoeniger, Edwin A. Saada
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Patent number: 11807877Abstract: The present invention relates, in part, to methods for detecting nuclease activity, such as the activity of Cas nucleases. Also described herein are compositions for conducting assays, as well as methods for conducting assays in the presence of test compounds.Type: GrantFiled: March 21, 2019Date of Patent: November 7, 2023Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Kyle Jeffrey Seamon, Brooke Nicole Harmon, Joseph S. Schoeniger, Yooli Kim Light
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Patent number: 11564919Abstract: An in vitro assay was designed to measure the activity of the alphavirus non-structural protein 2 (nsP2), which is the viral protease and is required for viral replication. By taking advantage of fluorescence-resonance energy transfer between two proteins, a protease cleavage assay was generated. This was utilized for high-throughput screening of 40,000 small molecules. Inhibitors were validated using cell-based assays to measure alphavirus infection and cytotoxicity. Certain compounds were then characterized for anti-viral efficacy in various cell lines in numerous assays. Compounds were tested against Chikungunya virus, Venezuelan Equine Encephalitis virus, Rift Valley Fever virus, and Zika virus. Three compounds (compounds I, II, and III) showed pan-alphavirus anti-viral efficacy at concentrations that did not result in cell toxicity. An additional compound, structure IV, showed broad spectrum inhibition of all viruses tested.Type: GrantFiled: March 2, 2021Date of Patent: January 31, 2023Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Brooke Nicole Harmon, Oscar Negrete, Joseph S. Schoeniger, Edwin A. Saada
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Patent number: 11518989Abstract: A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.Type: GrantFiled: September 30, 2020Date of Patent: December 6, 2022Assignees: National Technology & Engineering Solutions of Sandia, LLC, Arizona Board of Regents on behalf of Arizona State UniversityInventors: Ryan Wesley Davis, Joseph S. Schoeniger, Arul M. Varman, Muhammad Faisal, Aditya Pandharinath Sarnaik
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Patent number: 11433121Abstract: A construct includes a core comprising an external surface and a plurality of pores, a cargo disposed in a pore of the plurality of pores, the cargo comprising a CRISPR Cas9 component or a nucleic acid sequence encoding a CRISPR Cas9 component; and a coating coupled to the core, wherein the coating comprises a cationic lipid, a pegylated lipid, a zwitterionic lipid, and a sterol. The coating may comprise a molar ratio of about 1 cationic lipid to 1 zwitterionic lipid to 0.9 sterol to 0.15 PEGylated lipid, wherein each molar ratio optionally varies by about plus or minus 10%. A method of treatment is also provided.Type: GrantFiled: April 3, 2020Date of Patent: September 6, 2022Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Oscar Negrete, Annette Estelle LaBauve, Joseph S. Schoeniger, Edwin A. Saada
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Patent number: 10267788Abstract: A method including identifying one or more structures in adjacent sequences of one or more proteins that can be mimicked by a peptide; and assembling a peptide that mimics the one or more secondary structures. A method including assembling epitopes of linked pairs of discontiguous peptide subsequences that mimic one or more structures in sequences of a protein; and assaying antibodies against the epitopes.Type: GrantFiled: January 20, 2014Date of Patent: April 23, 2019Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Joseph S. Schoeniger, Peter Anderson
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Patent number: 9024111Abstract: The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.Type: GrantFiled: March 30, 2012Date of Patent: May 5, 2015Assignee: Sandia CorporationInventors: Joseph S. Schoeniger, Masood Zia Hadi
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Patent number: 7368290Abstract: The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination. Additionally, the preferred method of the present invention involves the determination of protein structures utilizing a top-down analysis of protein structures to search for covalent modifications. In the preferred method, intact proteins are ionized and fragmented within the mass spectrometer.Type: GrantFiled: May 12, 2003Date of Patent: May 6, 2008Assignee: Sandia National LaboratoriesInventors: Gary Kruppa, Joseph S. Schoeniger, Malin M. Young
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Patent number: 6960285Abstract: An electrokinetic pump capable of producing high pressure is combined with a nozzle having a submicron orifice to provide a high pressure spray device. Because of its small size, the device can be contained within medical devices such as an endoscope for delivering biological materials such as DNA, chemo therapeutic agents, or vaccines to tissues and cells.Type: GrantFiled: September 30, 2002Date of Patent: November 1, 2005Assignee: Sandia Naitonal LaboratoriesInventors: Joseph S. Schoeniger, Phillip H. Paul, Luke Schoeniger
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Patent number: 6843272Abstract: A device for interrupting or throttling undesired ionic transport through a fluid network is disclosed. The device acts as a fluid valve by reversibly generating a fixed “bubble” in the conducting solvent solution carried by the network. The device comprises a porous hydrophobic structure filling a portion of a connecting channel within the network and optionally incorporates flow restrictor elements at either end of the porous structure that function as pressure isolation barriers, and a fluid reservoir connected to the region of the channel containing the porous structure. Also included is a pressure pump connected to the fluid reservoir. The device operates by causing the pump to vary the hydraulic pressure to a quantity of solvent solution held within the reservoir and porous structure. At high pressures, most or all of the pores of the structure are filled with conducting liquid so the ionic conductance is high.Type: GrantFiled: November 25, 2002Date of Patent: January 18, 2005Assignee: Sandia National LaboratoriesInventors: Joseph S. Schoeniger, Eric B. Cummings, James S. Brennan
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Publication number: 20040229369Abstract: The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination.Type: ApplicationFiled: May 12, 2003Publication date: November 18, 2004Inventors: Gary Kruppa, Joseph S. Schoeniger
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Publication number: 20040099321Abstract: A device for interrupting or throttling undesired ionic transport through a fluid network is disclosed. The device acts as a fluid valve by reversibly generating a fixed “bubble” in the conducting solvent solution carried by the network. The device comprises a porous hydrophobic structure filling a portion of a connecting channel within the network and optionally incorporates flow restrictor elements at either end of the porous structure that function as pressure isolation barriers, and a fluid reservoir connected to the region of the channel containing the porous structure. Also included is a pressure pump connected to the fluid reservoir. The device operates by causing the pump to vary the hydraulic pressure to a quantity of solvent solution held within the reservoir and porous structure. At high pressures, most or all of the pores of the structure are filled with conducting liquid so the ionic conductance is high.Type: ApplicationFiled: November 25, 2002Publication date: May 27, 2004Inventors: Joseph S. Schoeniger, Eric B. Cummings, James S. Brennan
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Patent number: 6572749Abstract: An electrokinetic high pressure hydraulic pump for manipulating fluids in capillary-based system. The pump uses electro-osmotic flow to provide a high pressure hydraulic system, having no moving mechanical parts, for pumping and/or compressing fluids, for providing valve means and means for opening and closing valves, for controlling fluid flow rate, and manipulating fluid flow generally and in capillary-based systems (microsystems), in particular. The compact nature of the inventive high pressure hydraulic pump provides the ability to construct a micro-scale or capillary-based HPLC system that fulfills the desire for small sample quantity, low solvent consumption, improved efficiency, the ability to run samples in parallel, and field portability. Control of pressure and solvent flow rate is achieved by controlling the voltage applied to an electrokinetic pump.Type: GrantFiled: July 23, 2001Date of Patent: June 3, 2003Assignee: Sandia CorporationInventors: Phillip H. Paul, David J. Rakestraw, Don W. Arnold, Kenneth R. Hencken, Joseph S. Schoeniger, David W. Neyer
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Publication number: 20030038027Abstract: An electrokinetic pump capable of producing high pressure is combined with a nozzle having a submicron orifice to provide a high pressure spray device. Because of its small size, the device can be contained within medical devices such as an endoscope for delivering biological materials such as DNA, chemo therapeutic agents, or vaccines to tissues and cells.Type: ApplicationFiled: September 30, 2002Publication date: February 27, 2003Inventors: Joseph S. Schoeniger, Phillip H. Paul, Luke Schoeniger
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Patent number: 6495015Abstract: An electrokinetic pump capable of producing high pressure is combined with a nozzle having a submicron orifice to provide a high pressure spray device. Because of its small size, the device can be contained within medical devices such as an endoscope for delivering biological materials such as DNA, chemo therapeutic agents, or vaccines to tissues and cells.Type: GrantFiled: June 16, 2000Date of Patent: December 17, 2002Assignee: Sandia National CorporationInventors: Joseph S. Schoeniger, Phillip H. Paul, Luke Schoeniger
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Patent number: 6428666Abstract: A method for separating and concentrating charged species from uncharged or neutral species regardless of size differential. The method uses reversible electric field induced retention of charged species, that can include molecules and molecular aggregates such as dimers, polymers, multimers, colloids, micelles, and liposomes, in volumes and on surfaces of porous materials. The retained charged species are subsequently quantitatively removed from the porous material by a pressure driven flow that passes through the retention volume and is independent of direction thus, a multi-directional flow field is not required. Uncharged species pass through the system unimpeded thus effecting a complete separation of charged and uncharged species and making possible concentration factors greater than 1000-fold.Type: GrantFiled: February 22, 1999Date of Patent: August 6, 2002Assignee: Sandia National LaboratoriesInventors: Anup K. Singh, David W. Neyer, Joseph S. Schoeniger, Michael G. Garguilo
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Patent number: 6287440Abstract: A method for eliminating gas bubble blockage of current flow during operation of an electrokinetic pump. By making use of the ability to modify the surface charge on the porous dielectric medium used in electrokinetic pumps, it becomes possible to place electrodes away from the pressurized region of the electrokinetic pump. While gas is still generated at the electrodes they are situated such that the generated gas can escape into a larger buffer reservoir and not into the high pressure region of the pump where the gas bubbles can interrupt current flow. Various combinations of porous dielectric materials and ionic conductors can be used to create pumps that have desirable electrical, material handling, and flow attributes.Type: GrantFiled: June 18, 1999Date of Patent: September 11, 2001Assignee: Sandia CorporationInventors: Don W. Arnold, Phillip H. Paul, Joseph S. Schoeniger
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Patent number: 6277257Abstract: An electrokinetic high pressure hydraulic pump for manipulating fluids in capillary-based systems. The pump uses electro-osmotic flow to provide a high pressure hydraulic system, having no moving mechanical parts, for pumping and/or compressing fluids, for providing valve means and means for opening and closing valves, for controlling fluid flow rate, and manipulating fluid flow generally and in capillary-based systems (Microsystems), in particular. The compact nature of the inventive high pressure hydraulic pump provides the ability to construct a micro-scale or capillary-based HPLC system that fulfills the desire for small sample quantity, low solvent consumption, improved efficiency, the ability to run samples in parallel, and field portability. Control of pressure and solvent flow rate is achieved by controlling the voltage applied to an electrokinetic pump.Type: GrantFiled: March 18, 1999Date of Patent: August 21, 2001Assignee: Sandia CorporationInventors: Phillip H. Paul, David J. Rakestraw, Don W. Arnold, Kenneth R. Hencken, Joseph S. Schoeniger, David W. Neyer
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Patent number: 6210986Abstract: A new channel structure for microfluidic systems and process for fabricating this structure. In contrast to the conventional practice of fabricating fluid channels as trenches or grooves in a substrate, fluid channels are fabricated as thin walled raised structures on a substrate. Microfluidic devices produced in accordance with the invention are a hybrid assembly generally consisting of three layers: 1) a substrate that can or cannot be an electrical insulator; 2) a middle layer, that is an electrically conducting material and preferably silicon, forms the channel walls whose height defines the channel height, joined to and extending from the substrate; and 3) a top layer, joined to the top of the channels, that forms a cover for the channels. The channels can be defined by photolithographic techniques and are produced by etching away the material around the channel walls.Type: GrantFiled: September 23, 1999Date of Patent: April 3, 2001Assignee: Sandia CorporationInventors: Don W. Arnold, Joseph S. Schoeniger, Gregory F. Cardinale