Patents by Inventor Josh Molho

Josh Molho has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9539574
    Abstract: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: January 10, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Charles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho
  • Patent number: 9034196
    Abstract: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.
    Type: Grant
    Filed: September 21, 2011
    Date of Patent: May 19, 2015
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Stephane Mouradian, Josh Molho, Ming Wu, Hayley Wu, Javier A. Farinas
  • Patent number: 9012206
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: April 21, 2015
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Publication number: 20150047980
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Application
    Filed: October 28, 2014
    Publication date: February 19, 2015
    Applicant: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Patent number: 8871524
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: October 28, 2014
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Patent number: 8834807
    Abstract: The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity<0.2 mS/cm. In a method, a buffer is loaded into a loading well in fluid communication with a separation channel of a device. A sample material having a conductivity higher than that of the buffer is then loaded into the loading well such that the sample material is disposed beneath the buffer, the buffer disposed over and covering the sample material. The sample material is separated into a plurality of separated components in the separation channel, and a separated component is collected from a collection well disposed in a collection leg of the device.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: September 16, 2014
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu
  • Patent number: 8545771
    Abstract: The invention provides fluidic devices having incorporated electrodes. One device comprises a card and first and second caddy segments. The first caddy segment comprises first and second electrodes. The second caddy segment comprises first and second reservoirs disposed on a first surface of the second segment, a channel disposed on a second surface of the second segment, and first and second vias extending between the first and second surfaces. The first caddy segment is attached to the first surface of the second caddy segment. The card is attached to the second surface of the second caddy segment such that the card provides a closed surface for the device.
    Type: Grant
    Filed: November 7, 2011
    Date of Patent: October 1, 2013
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Colin B. Kennedy, Josh Molho, Alexander V. Dukhovny
  • Patent number: 8367021
    Abstract: The invention provides fluidic devices having incorporated electrodes. One device comprises a card and a caddy. The card includes a channel disposed within the card, first and second vias in fluid communication with the channel through an upper surface of the card, and first and second electrodes disposed on the upper surface of the card. The first via and first electrode are positioned adjacent to a first end of the channel, and the second via and second electrode are positioned adjacent to a second end of the channel. The caddy comprises first and second reservoirs. The caddy is attached to the card such that the first reservoir is positioned over the first via and a portion of the first electrode, and the second reservoir is positioned over the second via and a portion of the second electrode. A portion of each of the electrodes is accessible for dry electrical contact.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: February 5, 2013
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Colin B. Kennedy, Josh Molho, Alexander V. Dukhovny
  • Patent number: 8277744
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well between first and second ends of the collection leg, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: October 2, 2012
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu, Jorge J. Zaninovich
  • Publication number: 20120222959
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well between first and second ends of the collection leg, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Application
    Filed: May 14, 2012
    Publication date: September 6, 2012
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Josh Molho, Hui Xu, Jorge J. Zaninovich
  • Patent number: 8202486
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device. and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: June 19, 2012
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu, Jorge J. Zaninovich
  • Publication number: 20120080315
    Abstract: The invention provides fluidic devices having incorporated electrodes, systems for using such devices, and methods for manufacturing such devices. The invention also provides fluidic devices configured to absorb joule heating within the device.
    Type: Application
    Filed: November 7, 2011
    Publication date: April 5, 2012
    Inventors: Colin B. Kennedy, Josh Molho, Alexander V. Dukhovny
  • Publication number: 20120053880
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Application
    Filed: November 1, 2011
    Publication date: March 1, 2012
    Applicant: Caliper Life Sciences, Inc.
    Inventors: DAN CAMPORESE, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Publication number: 20120043206
    Abstract: The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity <0.2 mS/cm. In a method, a buffer is loaded into a loading well in fluid communication with a separation channel of a device. A sample material having a conductivity higher than that of the buffer is then loaded into the loading well such that the sample material is disposed beneath the buffer, the buffer disposed over and covering the sample material. The sample material is separated into a plurality of separated components in the separation channel, and a separated component is collected from a collection well disposed in a collection leg of the device.
    Type: Application
    Filed: November 1, 2011
    Publication date: February 23, 2012
    Applicant: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu
  • Publication number: 20120009098
    Abstract: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.
    Type: Application
    Filed: September 21, 2011
    Publication date: January 12, 2012
    Applicant: Caliper Life Sciences, Inc.
    Inventors: Stephane Mouradian, Josh Molho, Ming Wu, Hayley Wu, Javier A. Farinas
  • Publication number: 20110139274
    Abstract: The invention provides fluidic devices having incorporated electrodes, systems for using such devices, and methods for manufacturing such devices. The invention also provides fluidic devices configured to absorb joule heating within the device.
    Type: Application
    Filed: February 18, 2011
    Publication date: June 16, 2011
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Colin B. Kennedy, Josh Molho, Alexander V. Dukhovny
  • Publication number: 20110036411
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device, and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Application
    Filed: July 26, 2010
    Publication date: February 17, 2011
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: JOSH MOLHO, HUI XU, JORGE J. ZANINOVICH
  • Publication number: 20110024296
    Abstract: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
    Type: Application
    Filed: October 11, 2010
    Publication date: February 3, 2011
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Charles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho
  • Publication number: 20080277356
    Abstract: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath.
    Type: Application
    Filed: May 7, 2007
    Publication date: November 13, 2008
    Applicant: Caliper Life Sciences, Inc.
    Inventors: Stephane Mouradian, Josh Molho, Ming Wu, Hayley Wu, Javier A. Farinas
  • Publication number: 20070184463
    Abstract: A method of purifying a biological component found in a biological sample by extracting the biological component from the biological sample. The method is performed using a microfluidic device having at least one well for receiving the biological sample and at least one channel for introducing and removing fluids. A plurality of magnetic beads having a factor with an affinity for the biological component is introduced to the well together with a suitable biological sample. The biological sample is manipulated to release the biological component in proximity to the magnetic beads which are then segregated within the well while removing the biological sample. An elution solution for the biological component is introduced to the well and the elution solution together with the biological component are withdrawn therefrom.
    Type: Application
    Filed: October 2, 2006
    Publication date: August 9, 2007
    Applicants: Caliper Life Sciences, Inc., Canon U.S. Life Sciences, Inc.
    Inventors: Josh Molho, Pamela Foreman