Abstract: Described herein are systems and methods used for washing needle assemblies with multiple needles.

Abstract: Described herein are systems and methods used for washing needle assemblies with multiple needles.

Abstract: A liquid chromatography sample manager includes a thermal chamber, a sample platter mounted in the thermal chamber, and a needle drive including a base having a shaft configured to rotate about a vertical axis, the base attachable to an interior of a sample manager of a liquid chromatography system. The needle drive further includes a needle assembly attached to the base, the needle assembly including a sample needle, and a drive system attached to the base, the drive system including a sample needle motor configured to impart vertical movement of the sample needle. The liquid chromatography sample manager further includes a sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

Abstract: A seal pack of a sample manager of a liquid chromatography system having a plurality of wash flow pathways fluidically connected to a central pathway that accommodates a sample needle, wherein a first wash flow pathway is vertically offset from a second wash flow pathway, such that a wash solution flows axially along an exterior surface of the sample needle in a vertical direction to wash the sample needle when flowing from the first wash flow pathway to the vertically offset second wash flow pathway, is provided. Furthermore, an autosampler and associated methods are also provided.

Abstract: A liquid chromatography sample manager includes a thermal chamber, a sample platter mounted in the thermal chamber, and a needle drive including a base having a shaft configured to rotate about a vertical axis, the base attachable to an interior of a sample manager of a liquid chromatography system. The needle drive further includes a needle assembly attached to the base, the needle assembly including a sample needle, and a drive system attached to the base, the drive system including a sample needle motor configured to impart vertical movement of the sample needle. The liquid chromatography sample manager further includes a sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

Abstract: A method and a fluidic network for acquiring and injecting a chromatographic sample into a chromatography system flow include a metering pump module, a sample needle, a needle seal and an injection valve. The metering pump module includes a metering pump and a pressure transducer in serial fluidic communication. When the injection valve is in a first valve state, the injection valve is configured to fluidically terminate ports of the metering pump module. When the injection valve is in a second valve state, the injection valve is configured to fluidically couple a fluidic path that includes the metering pump module and sample needle into the system flow of a chromatography system without resulting in a substantial change in the pressure of the system flow.

Abstract: A method for injecting a sample into a flow of a liquid chromatography system includes providing a flow of a mobile phase, and injecting a volume of a sample into the flow of the mobile phase such that the concentration of the sample within the mobile phase varies with a triangular profile over the course of the injecting.

Abstract: A liquid chromatography sample manager includes a sampling mechanism; a sample platter mounted in the sampling mechanism, the sample platter configured to rotate about a first vertical axis; a needle arm mounted within the sampling mechanism, the needle arm configured to rotate about a second vertical axis; and a sample delivery system in fluidic communication with solvent delivery system, the sample delivery system including a sample needle attached to the needle arm, the sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

Abstract: A method and a fluidic network for acquiring and injecting a chromatographic sample into a chromatography system flow include a metering pump module, a sample needle, a needle seal and an injection valve. The metering pump module includes a metering pump and a pressure transducer in serial fluidic communication. When the injection valve is in a first valve state, the injection valve is configured to fluidically terminate ports of the metering pump module. When the injection valve is in a second valve state, the injection valve is configured to fluidically couple a fluidic path that includes the metering pump module and sample needle into the system flow of a chromatography system without resulting in a substantial change in the pressure of the system flow.

Abstract: Described is a metering pump that may be used to meter volumes of sample in a chromatography system. The metering pump has a modular configuration that allows for separation of a head pod from a drive assembly for easy serviceability. The head pod includes a pump head, cartridge housing, seal wash housing and plunger. The drive assembly can be implemented in an inline drive configuration. Alternatively, the drive assembly can be implemented in an offset drive configuration in which a stepper motor is displaced laterally from other drive assembly components but is coupled through a belt and pulley system.

Abstract: A seal pack of a sample manager of a liquid chromatography system having a plurality of wash flow pathways fluidically connected to a central pathway that accommodates a sample needle, wherein a first wash flow pathway is vertically offset from a second wash flow pathway, such that a wash solution flows axially along an exterior surface of the sample needle in a vertical direction to wash the sample needle when flowing from the first wash flow pathway to the vertically offset second wash flow pathway, is provided. Furthermore, an autosampler and associated methods are also provided.

Abstract: A seal pack of a sample manager of a liquid chromatography system having a plurality of wash flow pathways fluidically connected to a central pathway that accommodates a sample needle, wherein a first wash flow pathway is vertically offset from a second wash flow pathway, such that a wash solution flows axially along an exterior surface of the sample needle in a vertical direction to wash the sample needle when flowing from the first wash flow pathway to the vertically offset second wash flow pathway, is provided. Furthermore, an autosampler and associated methods are also provided.

Abstract: Described herein are systems and methods used for washing needle assemblies with multiple needles.

Abstract: Described are a fraction collector and a method of fraction collection for a liquid chromatography system. The method includes diverting a liquid chromatography system flow from a waste channel to a collection tube at a start of a fraction collection window and collecting the system flow dispensed from the collection tube during the fraction collection window. At the end of the window, the system flow is diverted to the waste channel and a flow of a wash solvent is provided to the collection tube to dispense liquid remaining in the collection tube at the end of the window from the collection tube. The method can increase the amount of the collected fraction and can reduce or eliminate cross-contamination of a subsequently collected fraction. The method is useful for analytical scale applications where the collected fractions may have volumes defined by a limited number of drops dispensed from the collection tube.

Abstract: A twist-lock compliant needle including a housing component surrounding a needle holder holding a rigidly mounted needle, the housing component including a biasing element disposed therein, wherein the biasing element compresses as a needle tip of the needle contacts a surface is provided. An associated method is also provided.

Abstract: Described is a metering pump that may be used to meter volumes of sample in a chromatography system. The metering pump has a modular configuration that allows for separation of a head pod from a drive assembly for easy serviceability. The head pod includes a pump head, cartridge housing, seal wash housing and plunger. The drive assembly can be implemented in an inline drive configuration. Alternatively, the drive assembly can be implemented in an offset drive configuration in which a stepper motor is displaced laterally from other drive assembly components but is coupled through a belt and pulley system.

Abstract: A liquid chromatography sample manager includes a thermal chamber, a sample platter mounted in the thermal chamber, and a needle drive including a base having a shaft configured to rotate about a vertical axis, the base attachable to an interior of a sample manager of a liquid chromatography system. The needle drive further includes a needle assembly attached to the base, the needle assembly including a sample needle, and a drive system attached to the base, the drive system including a sample needle motor configured to impart vertical movement of the sample needle. The liquid chromatography sample manager further includes a sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

Abstract: A liquid chromatography sample manager includes a sampling mechanism; a sample platter mounted in the sampling mechanism, the sample platter configured to rotate about a first vertical axis; a needle arm mounted within the sampling mechanism, the needle arm configured to rotate about a second vertical axis; and a sample delivery system in fluidic communication with solvent delivery system, the sample delivery system including a sample needle attached to the needle arm, the sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

Abstract: A method for injecting a sample into a flow of a liquid chromatography system includes providing a flow of a mobile phase, and injecting a volume of a sample into the flow of the mobile phase such that the concentration of the sample within the mobile phase varies with a triangular profile over the course of the injecting.

Abstract: Described are a method and a fluidic network for acquiring and injecting a chromatographic sample into a chromatography system flow. The fluidic network includes a metering pump module, a sample needle, a needle seal and an injection valve. The metering pump module includes a metering pump and a pressure transducer in serial fluidic communication. When the injection valve is in a first valve state, the injection valve is configured to fluidically terminate ports of the metering pump module. When the injection valve is in a second valve state, the injection valve is configured to fluidically couple a fluidic path that includes the metering pump module and sample needle into the system flow of a chromatography system without resulting in a substantial change in the pressure of the system flow.