Abstract: The present invention provides a method of detecting one or more analytes in a target sample, the method comprising: a. providing a nanoparticle dimer adapted to bind the analyte; b. causing the dimer to pass through a nanopore by voltage-driven translocation; c. observing changes in the translocation current; and d. comparing the translocation current profile of the target sample to the translocation current profile of a control sample; wherein a change in the translocation current profile of the target sample versus the control sample indicates the presence of the analyte in the target sample. Also provided is a method of detecting one or more analytes in a target sample, the method comprising: a. providing a nanoparticle adapted to bind the analyte; b. providing a carrier nucleic acid molecule with at least one single-stranded region; c. contacting the carrier nucleic acid molecule and nanoparticle with the target sample, forming a carrier nucleic acid/analyte/nanoparticle complex; b.

Abstract: The disclosure relates to an apparatus and associated method for concentration of polarizable molecules within a fluid medium. The apparatus comprising a structure defining a cavity, having a cross-sectional dimension of 200 nm or less; at least two translocation electrodes positioned relative to the structure to enable generation of a DC electric field passing through the cavity; and at least two trapping electrodes positioned relative to the structure to enable generation of a time-varying electric field proximal to the cavity inlet.

Abstract: The invention relates to methods of detecting and/or quantifying analytes in a sample, as well as methods of detecting mutations and/or polymorphisms in nucleic acid molecules.

Abstract: THE present application relates to a method of detecting one or more analytes in a sample, the method comprising (a) providing a carrier nucleic acid molecule with at least one single-stranded region; (b) providing one or more aptamers specific for the analyte, wherein the aptamers additionally comprise a single-stranded portion complementary to at least one single-stranded region on the carrier nucleic acid; (c) contacting the carrier nucleic acid and one or more aptamers with the sample, forming a carrier nucleic acid/N aptamer/analyte complex, and; (d) detecting the presence of the carrier nucleic acid/aptamer/analyte complex.

Abstract: THE present application relates to a method of detecting one or more analytes in a sample, the method comprising (a) providing a carrier nucleic acid molecule with at least one single-stranded region; (b) providing one or more aptamers specific for the analyte, wherein the aptamers additionally comprise a single-stranded portion complementary to at least one single-stranded region on the carrier nucleic acid; (c) contacting the carrier nucleic acid and one or more aptamers with the sample, forming a carrier nucleic acid/N aptamer/analyte complex, and; (d) detecting the presence of the carrier nucleic acid/aptamer/analyte complex.

Abstract: The disclosure relates to an apparatus and associated method for concentration of polarizable molecules within a fluid medium. The apparatus comprising a structure defining a cavity, having a cross-sectional dimension of 200 nm or less; at least two translocation electrodes positioned relative to the structure to enable generation of a DC electric field passing through the cavity; and at least two trapping electrodes positioned relative to the structure to enable generation of a time-varying electric field proximal to the cavity inlet.

Abstract: The invention relates to a microfluidic system for generating droplets, the controlled merging of two or more droplets, and the analysis of droplets.

Abstract: The invention relates to a method of detecting the presence of an analyte associated with a nanoparticle layer formed at a liquid-liquid interface. The method comprises removing a portion of one of the liquid phases; and detecting the presence of the analyte by Raman spectroscopy, Infra Red spectroscopy and/or fluorescence spectroscopy.

Abstract: The invention provides methods of purifying one or more biological analytes from a sample. The method comprises incubating the sample with nanoparticles and centrifuging a biphasic system. The biological analytes can be one or more of an enzyme, a protein, a nucleic acid, an organelle, a cell, a bacteria, a virus, or any other biological material which requires purification.