Abstract: Provided herein are methods, oligonucleotides, and compositions for modulating levels of at least one target protein in a cell, e.g., by reducing levels of the mRNA of the target protein or inhibiting translation of the mRNA. In some embodiments, the target protein can be the ubiquitin ligase polypeptide, including, but not limited to WWP1, WWP2, and NEDD4. Also provided herein are methods, oligonucleotides, and compositions for treating, ameliorating, delaying or reducing a symptom of a cancer. The present disclosure also relates to compounds, methods and compositions for modulating at least one target protein selected from ubiquitin protein ligase. Also provided herein are compounds, methods, and compositions for treating, ameliorating, delaying or reducing a symptom of a cancer or a viral infection. In particular, the present disclosure is directed to specific small-molecule modulators of HECT domain E3 ligases that are useful in the therapy and diagnosis of cancer and viral infection.

Abstract: A method for detecting violation of a driving-related law includes producing, by at least one camera, at least one image of a vehicle. The method includes analyzing, by an analysis engine, the at least one image. The analysis engine identifies, based on analyzing the at least one image, a physical position of a driver of the vehicle within the vehicle and identifies a direction of a gaze of the driver. The analysis engine determines that at least one of the physical position of the driver within the vehicle and the direction of the gaze of the driver are associated with a violation of a driving-related law. The method includes transmitting, by a citation mananagement agement component, a notification of the association between the at least one of the physical position of the driver and the direction of the gaze of the driver and the violation, based upon the determination.