Abstract: Some embodiments provided herein relate to gene delivery systems and methods using a single plasmid that carries a self-inactivating transposase gene and a corresponding transposon. Some embodiments include nucleic acids having certain sequences, vector including such nucleic acids, and compositions including the vectors.

Abstract: Some embodiments provided herein relate to methods and compositions for making genetically modified T cells. In some such embodiments, CD4+ and CD8+ T cells are cultured in a single serum-free volume. In some embodiments, co-cultured CD4+ and CD8+ T cells can be transduced with a lentiviral vector, and a population of transduced T cells can be harvested within a shorter period of time than other conventional methods.

Abstract: A delivery system that includes a recombinantly synthesized protein polymer with protease cleavage sites such as matrix metalloproteinase responsive sequences engineered within the protein polymer. The system may be used to treat cancer, wounds, or pathological conditions in other tissues that express excess protease relative to healthy tissue.

Abstract: A delivery system that includes a recombinantly synthesized protein polymer with protease cleavage sites such as matrix metalloproteinase responsive sequences engineered within the protein polymer. The system may be used to treat cancer, wounds, or pathological conditions in other tissues that express excess protease relative to healthy tissue.

Abstract: A system and methods of incrementally updating a recording schedule in response to changes in user requests and changes in availability of requested content. Changes to the recording schedule may be prompted by a user request to record new content, a change in the parameters of an existing request, removal of a request, and the like. Before changing the recording schedule, an algorithm generates new possible recording schedules by combining recording assignments for a given program with the existing recording schedule. In one embodiment, the algorithm reduces the new possible recording schedules to a final recording schedule and applies the final recording schedule to a digital recording device. In another embodiment, the final recording schedule is presented to a user for approval before the schedule is applied to the digital recording device.

Abstract: Described herein is technology for, among other things, prioritizing video acquisition. The video acquisition may be prioritized implicitly, explicitly or a combination of both. Implicit prioritization involves receiving a request to acquire a video program. An attribute of the program is then analyzed to determine its value. The acquisition prioritization is then set based on this value. With explicit prioritization, the user is allowed to manually set the acquisition prioritization. In either case, future programs are then acquired based on the acquisition prioritization.