Patents by Inventor Joshua J. Coon

Joshua J. Coon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10852306
    Abstract: Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: December 1, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Michael S. Westphall
  • Patent number: 10809267
    Abstract: Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: October 20, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Michael S. Westphall
  • Patent number: 10273511
    Abstract: Enzymes for producing non-straight-chain fatty acids, microorganisms comprising the enzymes, and in vivo and in vitro uses of the enzymes. Provided are enzymes capable of producing various non-straight-chain fatty acids, including branched-chain fatty acids, cyclic fatty acids, and furan-containing fatty acids. The enzymes include RSP2144, RSP1091, and RSP1090 from Rhodobacter sphaeroides and homologs thereof. The enzymes can be purified to produce non-straight-chain fatty acids in vitro or expressed in microorganisms to produce non-straight-chain fatty acids in vivo. The microorganisms can be fine-tuned to produce a specific type of non-straight-chain fatty acid by expressing, overexpressing, or deleting the enzymes in various combinations.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: April 30, 2019
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Rachelle A. S. Lemke, Timothy James Donohue, Joshua J. Coon, Amelia C. Peterson, Michael S. Westphall
  • Patent number: 10153146
    Abstract: The invention provides methods, systems and algorithms for identifying high-resolution mass spectra. In some embodiments, an analyte is ionized and analyzed using high-resolution mass spectrometry (MS) at high mass accuracy (such as ?75 ppm or ?30 ppm) and the obtained mass spectra are matched with one or more prospective candidate molecules or chemical formulas. The invention provide, for example, methods and systems wherein the possible fragments that can be generated from the candidate molecules or chemical formulas are determined as well as the masses of each of these fragments. The invention provide, for example, methods and systems wherein the high-resolution mass spectra are then compared with the calculated fragment masses for each of the candidate molecules or chemical formula, and the portion of the high-resolution mass spectra that corresponds or can be explained by the calculated fragment masses is determined.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: December 11, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Nicholas W. Kwiecien, Derek J. Bailey, Michael S. Westphall, Joshua J. Coon
  • Publication number: 20170285042
    Abstract: Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
    Type: Application
    Filed: June 1, 2017
    Publication date: October 5, 2017
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. COON, Michael S. WESTPHALL
  • Patent number: 9698001
    Abstract: Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.
    Type: Grant
    Filed: April 3, 2012
    Date of Patent: July 4, 2017
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Michael S. Westphall
  • Patent number: 9478405
    Abstract: Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows MSn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells. Following injection and selection of a particular ion type or population, that population can be fragmented via beam-type collision-activated dissociation (CAD), as well as other dissociation methods, using the pre-existing ion injection pathway or inlet of a mass spectrometer. For CAD applications, this is achieved by transmitting the ions back along the ion injection pathway with a high degree of kinetic energy. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped.
    Type: Grant
    Filed: May 4, 2015
    Date of Patent: October 25, 2016
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Graeme C. McAlister
  • Publication number: 20160305953
    Abstract: The invention provides mass spectrometry methods, compositions and systems which enable a unique platform for analyte quantitation accessing very high degrees of multiplexing and accurate quantification, particularly well-suited for a range of quantitative analysis for proteomics applications. Embodiments of the present methods and systems combine isotopic coding agents characterized by very small differences in molecular mass with mass spectrometry methods providing large resolving power to provide relative or absolute analyte quantification in a large number of samples.
    Type: Application
    Filed: May 13, 2016
    Publication date: October 20, 2016
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. COON, Alex HEBERT
  • Patent number: 9366678
    Abstract: The invention provides mass spectrometry methods, compositions and systems which enable a unique platform for analyte quantitation accessing very high degrees of multiplexing and accurate quantification, particularly well-suited for a range of quantitative analysis for proteomics applications. Embodiments of the present methods and systems combine isotopic coding agents characterized by very small differences in molecular mass with mass spectrometry methods providing large resolving power to provide relative or absolute analyte quantification in a large number of samples.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: June 14, 2016
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J Coon, Alex Hebert
  • Publication number: 20160020081
    Abstract: Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows MSn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells. Following injection and selection of a particular ion type or population, that population can be fragmented via beam-type collision-activated dissociation (CAD), as well as other dissociation methods, using the pre-existing ion injection pathway or inlet of a mass spectrometer. For CAD applications, this is achieved by transmitting the ions back along the ion injection pathway with a high degree of kinetic energy. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped.
    Type: Application
    Filed: May 4, 2015
    Publication date: January 21, 2016
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. COON, Graeme C. MCALISTER
  • Publication number: 20150376659
    Abstract: Enzymes for producing non-straight-chain fatty acids, microorganisms comprising the enzymes, and in vivo and in vitro uses of the enzymes. Provided are enzymes capable of producing various non-straight-chain fatty acids, including branched-chain fatty acids, cyclic fatty acids, and furan-containing fatty acids. The enzymes include RSP2144, RSP1091, and RSP1090 from Rhodobacter sphaeroides and homologs thereof. The enzymes can be purified to produce non-straight-chain fatty acids in vitro or expressed in microorganisms to produce non-straight-chain fatty acids in vivo. The microorganisms can be fine-tuned to produce a specific type of non-straight-chain fatty acid by expressing, overexpressing, or deleting the enzymes in various combinations.
    Type: Application
    Filed: June 30, 2015
    Publication date: December 31, 2015
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Rachelle A.S. Lemke, Timothy James Donohue, Joshua J. Coon, Amelia C. Peterson, Michael S. Westphall
  • Publication number: 20150340216
    Abstract: The invention provides methods, systems and algorithms for identifying high-resolution mass spectra. In some embodiments, an analyte is ionized and analyzed using high-resolution mass spectrometry (MS) at high mass accuracy (such as ?75 ppm or ?30 ppm) and the obtained mass spectra are matched with one or more prospective candidate molecules or chemical formulas. The invention provide, for example, methods and systems wherein the possible fragments that can be generated from the candidate molecules or chemical formulas are determined as well as the masses of each of these fragments. The invention provide, for example, methods and systems wherein the high-resolution mass spectra are then compared with the calculated fragment masses for each of the candidate molecules or chemical formula, and the portion of the high-resolution mass spectra that corresponds or can be explained by the calculated fragment masses is determined.
    Type: Application
    Filed: March 27, 2015
    Publication date: November 26, 2015
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Nicholas W. KWIECIEN, Derek J. BAILEY, Michael S. WESTPHALL, Joshua J. COON
  • Patent number: 9053916
    Abstract: Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows MSn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells. Following injection and selection of a particular ion type or population, that population can be fragmented via beam-type collision-activated dissociation (CAD), as well as other dissociation methods, using the pre-existing ion injection pathway or inlet of a mass spectrometer. For CAD applications, this is achieved by transmitting the ions back along the ion injection pathway with a high degree of kinetic energy. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: June 9, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Graeme C. McAlister
  • Patent number: 9040903
    Abstract: Described herein are mass spectrometry systems and methods which utilize a dynamic a new data acquisition/instrument control methodology. These systems and methods employ novel artificial intelligence algorithms to greatly increase quantitative and/or identification accuracy during data acquisition. In an embodiment, the algorithms can adapt the instrument methods and systems during data acquisition to direct data acquisition resources to increase quantitative or identification accuracy of target analytes, such as proteins, peptides, and peptide fragments.
    Type: Grant
    Filed: April 3, 2012
    Date of Patent: May 26, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Michael S. Westphall, Graeme McAlister, Derek Bailey
  • Publication number: 20140246577
    Abstract: Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows MSn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells. Following injection and selection of a particular ion type or population, that population can be fragmented via beam-type collision-activated dissociation (CAD), as well as other dissociation methods, using the pre-existing ion injection pathway or inlet of a mass spectrometer. For CAD applications, this is achieved by transmitting the ions back along the ion injection pathway with a high degree of kinetic energy. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 4, 2014
    Inventors: Joshua J. COON, Graeme C. MCALISTER
  • Patent number: 8742333
    Abstract: Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. Following injection and selection of a particular ion type or population, that population can be fragmented using the pre-existing ion injection pathway or inlet of a mass spectrometer. This is achieved by transmitting the ions back along the ion injection pathway. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped. Following fragmentation and trapping, the ions can either be re-injected into the primary ion selection device or sent on to a secondary mass analyzer.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: June 3, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Graeme C. McAlister
  • Publication number: 20140120565
    Abstract: The invention provides mass spectrometry methods, compositions and systems which enable a unique platform for analyte quantitation accessing very high degrees of multiplexing and accurate quantification, particularly well-suited for a range of quantitative analysis for proteomics applications. Embodiments of the present methods and systems combine isotopic coding agents characterized by very small differences in molecular mass with mass spectrometry methods providing large resolving power to provide relative or absolute analyte quantification in a large number of samples.
    Type: Application
    Filed: October 25, 2012
    Publication date: May 1, 2014
    Inventors: Joshua J. COON, Alex HEBERT
  • Patent number: 8692187
    Abstract: The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.
    Type: Grant
    Filed: March 16, 2012
    Date of Patent: April 8, 2014
    Inventors: Donald F. Hunt, Joshua J. Coon, John Edward Philip Syka, Jarod A. Marto
  • Patent number: 8592216
    Abstract: The present invention provides methods for enhancing the fragmentation of peptides for mass spectrometry by modifying the peptides with a tagging reagent containing a functional group, such as a tertiary amine, having a greater gas-phase basicity than the amide backbone of the peptide. These high gas-phase basicity functional groups are attached to a peptide by reacting the tagging reagent to one or more available carboxylic acid groups of the peptide. Linking these high gas-phase functional groups to the peptides leads to higher charge state ions from electrospray ionization mass spectrometry (ESI-MS), which fragment more extensively during fragmentation techniques, particularly non-ergodic fragmentation techniques such as electron capture dissociation (ECD) and electron transfer dissociation (ETD).
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: November 26, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian L. Frey, April L. Jue, Casey J. Krusemark, Lloyd M. Smith, Joshua J. Coon
  • Patent number: 8563777
    Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: October 22, 2013
    Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the University of Illinois
    Inventors: Lloyd M. Smith, Michael R. Shortreed, Brian L. Frey, Margaret F. Phillips, Joshua J. Coon, Shane M. Lamos, Casey J. Krusemark, Peter J. Belshaw, Madhusudan Patel, Neil L. Kelleher