Abstract: Systems, apparatuses, and methods are described for 3D luminescence imaging, by identifying a preferred optical pair and optimizing a scanned image using the preferred optical pair. An optimal filter pair may be selected from a list of two or more optical filters. An acceptable threshold of information may be obtained using a subset of the list of two or more optical filters (e.g., an optimal filter pair). An imaging device may be configured with the optimal filter pair to produce a pair of luminescence images of a target sample. In addition, luminescence images may be pre-processed to reduce the time-cost of conventional processing techniques of luminescence images. One or more computing devices may generate initial prior data based on a pair of luminescence images. An output may include one or more output luminescent sources that have been refined and/or optimized from the initial prior data.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: Systems, apparatuses, and methods are described for 3D luminescence imaging, by identifying a preferred optical pair and optimizing a scanned image using the preferred optical pair. An optimal filter pair may be selected from a list of two or more optical filters. An acceptable threshold of information may be obtained using a subset of the list of two or more optical filters (e.g., an optimal filter pair). An imaging device may be configured with the optimal filter pair to produce a pair of luminescence images of a target sample. In addition, luminescence images may be pre-processed to reduce the time-cost of conventional processing techniques of luminescence images. One or more computing devices may generate initial prior data based on a pair of luminescence images. An output may include one or more output luminescent sources that have been refined and/or optimized from the initial prior data.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: Aspects of the present disclosure provide systems, methods, devices, and computer-readable media for interference filter correction based on angle of incidence. In some examples, a sample emits an emission spectrum that is filtered by an emission filter to provide a transmission spectrum. The emission spectrum illuminates the emission filter at multiple angles of incidence. The angles of incidence result in a spectral shifting of the transmission spectrum. Based on this spectral shifting, the intensity of the transmission spectrum is corrected. An image corresponding to the corrected intensity of the transmission spectrum may be generated.

Abstract: Aspects of the present disclosure provide systems, methods, devices, and computer-readable media for interference filter correction based on angle of incidence. In some examples, a sample emits an emission spectrum that is filtered by an emission filter to provide a transmission spectrum. The emission spectrum illuminates the emission filter at multiple angles of incidence. The angles of incidence result in a spectral shifting of the transmission spectrum. Based on this spectral shifting, the intensity of the transmission spectrum is corrected. An image corresponding to the corrected intensity of the transmission spectrum may be generated.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: The invention relates to systems and methods for tomographic imaging of a subject comprising diffuse media by converting measurements of electromagnetic radiation, e.g., fluorescent light, obtained in free space exterior to the subject into data that would be measured if the subject were surrounded by an infinite and homogeneous diffusive medium, e.g., a medium with optical properties equal to the average optical properties of the subject. After applying a transformation to convert measurements to virtually-matched values, propagation of light is simulated from the index-matched surface to a set of virtual detectors exterior to the subject and arranged in a geometrically advantageous fashion, for example, in a planar array, thereby facilitating the use of fast reconstruction techniques.

Abstract: Presented herein are efficient and reliable systems and methods for calculating and extracting three-dimensional central axes of bones of animal subjects—for example, animal subjects scanned by in vivo or ex vivo microCT platforms—to capture both the general and localized tangential directions of the bone, along with its shape, form, curvature, and orientation. With bone detection and segmentation algorithms, the skeletal bones of animal subjects scanned by CT or microCT scanners can be detected, segmented, and visualized. Three dimensional central axes determined using these methods provide important information about the skeletal bones.

Abstract: In certain embodiments, the invention relates to systems and methods for altering an image to compensate for variation in one or more physical and/or supervenient properties (e.g., optical absorption and/or scattering) in heterogeneous, diffuse tissue, thereby attenuating the effects of tissue waveguiding. The methods enable the proper identification of emission image regions that evidence waveguiding of electromagnetic radiation, and enables compensation of emission images for such waveguiding. The methods preserve the depth localization accuracy of the FMT approach and improve optical reconstruction in the targeted areas while eliminating spurious components of fluorescence from the acquired data set. Calibration methods for probe concentration mapping are also presented.

Abstract: In certain embodiments, the invention relates to systems and methods for altering an image to compensate for variation in one or more physical and/or supervenient properties (e.g., optical absorption and/or scattering) in heterogeneous, diffuse tissue, thereby attenuating the effects of tissue waveguiding. The methods enable the proper identification of emission image regions that evidence waveguiding of electromagnetic radiation, and enables compensation of emission images for such waveguiding. The methods preserve the depth localization accuracy of the FMT approach and improve optical reconstruction in the targeted areas while eliminating spurious components of fluorescence from the acquired data set. Calibration methods for probe concentration mapping are also presented.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: In certain embodiments, the invention relates to systems and methods for altering an image to compensate for variation in one or more physical and/or supervenient properties (e.g., optical absorption and/or scattering) in heterogeneous, diffuse tissue, thereby attenuating the effects of tissue waveguiding. The methods enable the proper identification of emission image regions that evidence waveguiding of electromagnetic radiation, and enables compensation of emission images for such waveguiding. The methods preserve the depth localization accuracy of the FMT approach and improve optical reconstruction in the targeted areas while eliminating spurious components of fluorescence from the acquired data set. Calibration methods for probe concentration mapping are also presented.

Abstract: In certain embodiments, the invention relates to systems and methods for altering an image to compensate for variation in one or more physical and/or supervenient properties (e.g., optical absorption and/or scattering) in heterogeneous, diffuse tissue, thereby attenuating the effects of tissue waveguiding. The methods enable the proper identification of emission image regions that evidence waveguiding of electromagnetic radiation, and enables compensation of emission images for such waveguiding. The methods preserve the depth localization accuracy of the FMT approach and improve optical reconstruction in the targeted areas while eliminating spurious components of fluorescence from the acquired data set. Calibration methods for probe concentration mapping are also presented.

Abstract: In certain embodiments, the invention relates to systems and methods for altering an image to compensate for variation in one or more physical and/or supervenient properties (e.g., optical absorption and/or scattering) in heterogeneous, diffuse tissue, thereby attenuating the effects of tissue waveguiding. The methods enable the proper identification of emission image regions that evidence waveguiding of electromagnetic radiation, and enables compensation of emission images for such waveguiding. The methods preserve the depth localization accuracy of the FMT approach and improve optical reconstruction in the targeted areas while eliminating spurious components of fluorescence from the acquired data set. Calibration methods for probe concentration mapping are also presented.