Patents by Inventor Joshua Kittleson

Joshua Kittleson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12122810
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Grant
    Filed: May 23, 2022
    Date of Patent: October 22, 2024
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Publication number: 20240294584
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Application
    Filed: December 8, 2023
    Publication date: September 5, 2024
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20240150416
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: June 26, 2023
    Publication date: May 9, 2024
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11858971
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Grant
    Filed: March 31, 2021
    Date of Patent: January 2, 2024
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11725030
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Grant
    Filed: March 15, 2022
    Date of Patent: August 15, 2023
    Assignee: BOLT THREADS, INC.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20230192965
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: October 10, 2022
    Publication date: June 22, 2023
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20230064786
    Abstract: The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.
    Type: Application
    Filed: August 11, 2022
    Publication date: March 2, 2023
    Inventors: David Breslauer, Joshua Kittleson, Loren Perelman, Lindsay Wray
  • Patent number: 11505654
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: November 22, 2022
    Assignee: BOLT THREADS, INC.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20220315630
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the a-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Application
    Filed: March 15, 2022
    Publication date: October 6, 2022
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20220306706
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Application
    Filed: May 23, 2022
    Publication date: September 29, 2022
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Patent number: 11447532
    Abstract: The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.
    Type: Grant
    Filed: September 14, 2017
    Date of Patent: September 20, 2022
    Assignee: BOLT THREADS, INC.
    Inventors: David Breslauer, Joshua Kittleson, Loren Perelman, Lindsay Wray
  • Patent number: 11370815
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Grant
    Filed: December 30, 2020
    Date of Patent: June 28, 2022
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Patent number: 11306127
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: April 19, 2022
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 11192982
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: December 7, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
  • Publication number: 20210340194
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Application
    Filed: March 31, 2021
    Publication date: November 4, 2021
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Publication number: 20210230235
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Application
    Filed: December 30, 2020
    Publication date: July 29, 2021
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Patent number: 10988515
    Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: April 27, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
  • Patent number: 10906947
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: February 2, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Publication number: 20200399328
    Abstract: Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having molecular weight of greater than about 10 kDal. Each repeat unit includes more than about 150 amino acid residues that are organized into a number of “quasi-repeat units.” The fibers have improved mechanical properties that better recapitulate those of the native silk fibers.
    Type: Application
    Filed: July 6, 2020
    Publication date: December 24, 2020
    Inventors: David BRESLAUER, Lindsay WRAY, Joshua KITTLESON
  • Publication number: 20200055996
    Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.
    Type: Application
    Filed: August 30, 2019
    Publication date: February 20, 2020
    Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray