Abstract: The present disclosure relates in some aspects to immunogenic compositions including recombinant peptides and proteins comprising coronavirus viral antigens and immunogens, e.g., coronavirus S protein peptides. In some aspects, the immunogenic composition comprises a secreted fusion protein comprising a soluble coronavirus viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. In some aspects, the immunogenic compositions provided herein are useful for generating an immune response, e.g., for treating or preventing a coronavirus infection. In some aspects, the immunogenic compositions provided herein may be used in a vaccine composition, e.g., as part of a prophylactic and/or therapeutic vaccine. Also provided herein are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: The present disclosure provides methods and compositions for affinity purification of proteins containing a C-terminal polypeptide sequence of a procollagen, such as trimeric fusion proteins. Also provided are polypeptides, fusion proteins, and recombinant polypeptides containing an Endo 180 ecto-domain or portion thereof capable of reversibly binding a C-terminal polypeptide sequence of a procollagen. Also provided are articles of manufacture, kits, and apparatus related to the Endo 180 ecto-domain polypeptides.

Abstract: Provided are immunogenic compositions including recombinant peptides and proteins comprising respiratory syncytial virus (RSV) viral antigens and immunogens, e.g., RSV F protein peptides. The immunogenic composition comprises a secreted fusion protein comprising a soluble RSV viral antigen joined by in-frame a disulfide bond-linked trimeric fusion protein. The immunogenic compositions are useful for generating an immune response, e.g., for treating or preventing an RSV infection. The immunogenic compositions may be used in a vaccine composition, e.g., as part of a prophylactic and/or therapeutic vaccine. Also provided herein are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: The present disclosure discloses recombinant peptides and proteins comprising coronavirus viral antigens and immunogens, e.g., coronavirus S protein peptides, useful for analyzing an analyte such as neutralizing antibodies. In some aspects, the recombinant peptides and proteins comprise a secreted fusion protein comprising a soluble coronavirus viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. Diagnostic methods and related kits are also disclosed.

Abstract: The present invention discloses immunogenic compositions including recombinant peptides and proteins comprising human immunodeficiency viruses (HIV) antigens and immunogens, e.g., gp 120 protein peptides. In some aspects, the immunogenic composition comprises a secreted fusion protein comprising a soluble HIV viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. In some aspects, the immunogenic compositions provided herein are useful for generating an immune response, e.g., for treating or preventing an HIV infection. In some aspects, the immunogenic compositions provided herein may be used in a vaccine composition, e.g., as part of a prophylactic and/or therapeutic vaccine. Also provided herein are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: Provided are immunogenic compositions comprising a secreted fusion protein, wherein the secreted fusion protein comprises a soluble influenza or rabies viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. Also provided are uses of the immunogenic compositions for generating an immune response against influenza or rabies infection and in a vaccine composition. Also provided are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: The present disclosure relates in some aspects to immunogenic compositions including recombinant peptides and proteins comprising coronavirus viral antigens and immunogens, e.g., coronavirus S protein peptides. In some aspects, the immunogenic composition comprises a secreted fusion protein comprising a soluble coronavirus viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. In some aspects, the immunogenic compositions provided herein are useful for generating an immune response, e.g., for treating or preventing a coronavirus infection. In some aspects, the immunogenic compositions provided herein may be used in a vaccine composition, e.g., as part of a prophylactic and/or therapeutic vaccine. Also provided herein are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: The present disclosure relates in some aspects to immunogenic compositions including recombinant peptides and proteins comprising coronavirus viral antigens and immunogens, e.g., coronavirus S protein peptides. In some aspects, the immunogenic composition comprises a secreted fusion protein comprising a soluble coronavirus viral antigen joined by in-frame fusion to a C-terminal portion of a collagen which is capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. In some aspects, the immunogenic compositions provided herein are useful for generating an immune response, e.g., for treating or preventing a coronavirus infection. In some aspects, the immunogenic compositions provided herein may be used in a vaccine composition, e.g., as part of a prophylactic and/or therapeutic vaccine. Also provided herein are methods for producing the recombinant peptides and proteins, prophylactic, therapeutic, and/or diagnostic methods, and related kits.

Abstract: A jitter measurement circuit includes an addition circuit to add a digital rectangular signal to an adjustment signal generated by a circuit that generates a first error signal based on a phase difference between a first clock signal or a data signal on which the first clock signal is superimposed and a second clock signal and generates the adjustment signal by filtering the first error signal, and a calculation circuit to calculate a first correlation value for representing an autocorrelation of the first error signal when the rectangular signal is not added to the adjustment signal, and a second correlation value for representing the autocorrelation when the rectangular signal is added to the adjustment signal, based on the first error signal and a second error signal obtained by delaying the first error signal by a variable delay amount, and output the first correlation value and the second correlation value.

Abstract: A serial-parallel conversion circuit includes: a phase detector that outputs a first phase detection signal indicating whether a phase of a clock signal is advance or behind, a signal amplifying circuit that amplifies the first phase detection signal with a gain so as to output a second phase detection signal; a control loop that adjusts the phase of the clock signal based on the second phase detection signal; an autocorrelation circuit that generates an autocorrelation value based on the first phase detection signal and a set delay amount, and outputs an autocorrelation signal indicating the autocorrelation value; a gain adjusting circuit that adjusts the gain in such a manner that the autocorrelation value matches a target correlation value; and a delay-amount determination circuit that sets a delay amount corresponding to a peak value of an obtained autocorrelation value obtained when the autocorrelation value changes in an oscillatory manner.

Abstract: A jitter measurement circuit includes an addition circuit to add a digital rectangular signal to an adjustment signal generated by a circuit that generates a first error signal based on a phase difference between a first clock signal or a data signal on which the first clock signal is superimposed and a second clock signal and generates the adjustment signal by filtering the first error signal, and a calculation circuit to calculate a first correlation value for representing an autocorrelation of the first error signal when the rectangular signal is not added to the adjustment signal, and a second correlation value for representing the autocorrelation when the rectangular signal is added to the adjustment signal, based on the first error signal and a second error signal obtained by delaying the first error signal by a variable delay amount, and output the first correlation value and the second correlation value.

Abstract: A serial-parallel conversion circuit includes: a phase detector that outputs a first phase detection signal indicating whether a phase of a clock signal is advance or behind, a signal amplifying circuit that amplifies the first phase detection signal with a gain so as to output a second phase detection signal; a control loop that adjusts the phase of the clock signal based on the second phase detection signal; an autocorrelation circuit that generates an autocorrelation value based on the first phase detection signal and a set delay amount, and outputs an autocorrelation signal indicating the autocorrelation value; a gain adjusting circuit that adjusts the gain in such a manner that the autocorrelation value matches a target correlation value; and a delay-amount determination circuit that sets a delay amount corresponding to a peak value of an obtained autocorrelation value obtained when the autocorrelation value changes in an oscillatory manner.