Abstract: The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for automated fluid transfer, comprising a fluid pump, a fluid device, comprising a container for a volume of fluid, and a universal collar couplable to the container, the collar comprising a plurality of conduits, a sterile liquid transfer port in fluid communication with the plurality of conduits, a fluid pump module comprising compressible fluidic tubing coupled between an inlet port and an outlet port, each of the inlet port and the outlet port being in fluid communication with the plurality of conduits, and one or more viewing windows, and one or more sensors configured to detect, via the one or more viewing windows, the presence of liquid within a segment of the plurality of conduits.

Abstract: Digital microfluidic (DMF) methods and apparatuses (including devices, systems, cartridges, DMF readers, etc.), and in particular DMF apparatuses and methods that may be used to safely manually add or remove fluid within a cartridge while it is actively applying DMF. Also described herein are DMF readers for use with a DMF cartridges, including those including multiple and/or redundant safety interlocks. Also described herein are DMF reader devices having a cover with active control of microfluidics on the cover while actively controlling DMF on the reader base.