Patents by Inventor Joshua SHIRLEY
Joshua SHIRLEY has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240101980Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: November 28, 2023Publication date: March 28, 2024Inventors: Daniel MAZUR, Sihong CHEN, Peter VANDER HORN, Eileen TOZER, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN
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Patent number: 11937864Abstract: The invention relates to a magnetic-navigation joint type puncture needle which comprises a head section and needle sections, wherein, the head section is made of stainless steel material with high magnetic conductivity and is connected with the following needle sections coaxially mounted with the head section through a flexible joint; two needle sections are also coaxially connected with each other through a flexible joint; the needle sections are made of non-magnetic stainless steel material; the flexible joints adopts serpentine tubes formed by melting and engraving cobalt-chromium alloy through laser; and both the head section and the needle sections can be bent, but the flexibility of the head section and that of the needle sections are smaller than the flexibility of the flexible joints.Type: GrantFiled: November 7, 2019Date of Patent: March 26, 2024Assignee: DFine, Inc.Inventors: Joshua Defosset, Nate Shirley, Eric Wong, Craig Purdy, Oleg Yurchak, Jimmy Chi-Yun Chan, Robert D. Poser
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Patent number: 11866740Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: GrantFiled: May 7, 2021Date of Patent: January 9, 2024Assignee: Life Technologies CorporationInventors: Daniel Mazur, Peter B. Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
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Publication number: 20210284976Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: May 7, 2021Publication date: September 16, 2021Inventors: Daniel MAZUR, Peter B. VANDER HORN, Eileen TOZER, Sihong CHEN, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN
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Patent number: 11001814Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: GrantFiled: May 17, 2019Date of Patent: May 11, 2021Assignee: Life Technologies CorporationInventors: Daniel Mazur, Peter Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
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Publication number: 20190270974Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: May 17, 2019Publication date: September 5, 2019Inventors: Daniel MAZUR, Peter VANDER HORN, Eileen TOZER, Sihong CHEN, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN
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Patent number: 10344268Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: GrantFiled: September 27, 2016Date of Patent: July 9, 2019Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Daniel Mazur, Peter Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
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Publication number: 20170096648Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: September 27, 2016Publication date: April 6, 2017Inventors: DANIEL MAZUR, Peter VANDER HORN, Eileen TOZER, Sihong CHEN, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN