Patents by Inventor Joshua Soldo

Joshua Soldo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for detecting 1,25-dihydroxyvitamin D and related antibodies
    Patent number: 11261257
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: March 1, 2022
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Use of 1,25-dihydroxyvitamin D values in ratio with PTH as a prognostic biomarker
    Patent number: 10983136
    Abstract: The present invention relates to the use of 1,25-dihydroxyvitamin D values in ratio with PTH as a prognostic biomarker. More particularly, the present invention relates to a method for predicting or stratifying the risk of worsening renal function (WRF) in a patient at risk of renal injury or affected by renal injury. Levels of 1,25-dihydroxyvitamin D (1,25(OH)2D) are measured in a biological sample and taken together with parathyroid hormone (PTH) levels to provide a ratio indicative of the risk of worsening renal function.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: April 20, 2021
    Assignee: DiaSorin S.p.A.
    Inventors: Francesco Colotta, Fabrizio Bonelli, Frank Blocki, Claudia Zierold, Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca
  • Methods for Detecting 1,25-dihydroxyvitamin D and Related Antibodies
    Publication number: 20200062852
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
    Type: Application
    Filed: November 12, 2019
    Publication date: February 27, 2020
    Applicant: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Methods for detecting 1,25-dihydroxyvitamin D and related antibodies
    Patent number: 10501548
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
    Type: Grant
    Filed: May 26, 2017
    Date of Patent: December 10, 2019
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Method and kit for detecting 1,25-dihydroxyvitamin D and related antibodies
    Patent number: 10196449
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method of the invention, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method of the invention. The assay of the invention is preferably a sandwich immunoassay.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: February 5, 2019
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Methods for Detecting 1,25-Dihydroxyvitamin D and Related Antibodies
    Publication number: 20170267770
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
    Type: Application
    Filed: May 26, 2017
    Publication date: September 21, 2017
    Applicant: Diasorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Method and Kit for Detecting 1,25-Dihydroxyvitamin D and Related Antibodies
    Publication number: 20150361178
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method of the invention, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method of the invention. The assay of the invention is preferably a sandwich immunoassay.
    Type: Application
    Filed: January 27, 2014
    Publication date: December 17, 2015
    Applicant: DIASORIN S.P.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli