Abstract: The invention relates to a method of predicting a response of a prostate cancer subject to radiotherapy, comprising determining or receiving the result of a determination of a gene expression profile for each of three or more interleukin genes selected from the group consisting of: IL17RE, IL1B, IL3, IL7R, IL9R, and EBI3, said gene expression profiles being determined in a biological sample obtained from the subject, and determining, preferably by a processor, the prediction of the radiotherapy response based on the gene expression profiles for the three or more interleukin genes.

Abstract: The invention relates to a method of predicting a response of a prostate cancer subject to radiotherapy, comprising determining or receiving the result of a determination of a gene expression profile for each of five or more immune defense response genes selected from the group consisting of: AIM2, APOBEC3A, CIAO1, DDX58, DHX9, IFI16, IFIH1, IFIT1, IFIT3, LRRFIP1, MYD88, OAS1, TLR8, and ZBP1, said gene expression profiles being determined in a biological sample obtained from the subject, and determining, by a processor, the prediction of the radiotherapy response based on the gene expression profiles for the five or more immune defense response genes.

Abstract: The invention relates to a method of predicting a response of a prostate cancer subject to radiotherapy, comprising determining or receiving the result of a determination of a gene expression profile for each of eight or more T-Cell receptor signaling genes selected from the group consisting of: CD2, CD247, CD28, CD3E, CD3G, CD4, CSK, EZR, FYN, LAT, LCK, PAG1, PDE4D, PRKACA, PRKACB, PTPRC, and ZAP70, said gene expression profiles being determined in a biological sample obtained from the subject, and determining, by a processor, the prediction of the radiotherapy response based on the gene expression profiles for the eight or more T-Cell receptor signaling genes.

Abstract: A sensor platform (300) with a sensor surface (350) receives particles (400). A material extending from the sensor surface (350) having a refractive index higher than the one of the fluid (200), such that an electromagnetic wave (10) propagating in this platform material (310) and incident to the sensor surface (350) at an angle greater than the critical optical angle is totally reflected onto the sensor surface (350). The particles (400) suspended in the fluid (200) include a metallic material (410) enabling a localized surface plasmon resonance at resonant wavelength(s). An optical detector (102) detects a portion of a spectrum of the totally reflected wave (20), including the resonant wavelength(s). A processor determines a presence of the particles (400) on or close to the sensor surface (350) from a frustrated totally internal reflection (“FTIR”) signal retrieved from the detected wavelengths. The retrieving takes into account the detected resonant component(s) present in the FTIR signal.

Abstract: The invention relates to a system for optically detecting particles (400) in a fluid (200). It comprises on the one hand a sensor platform (300) with a sensor surface (350) to receive said particles (400) and a material extending from the sensor surface (350) having a refractive index higher than the one of the fluid (200), such that an electromagnetic wave (10) propagating in this platform material (310) and incident to the sensor surface (350) at an angle greater than the critical optical angle is totally reflected onto the sensor surface (350). Said particles (400) comprise a metallic material (410) enabling a localized surface plasmon resonance at least one resonant wavelength(s) if excited at excitation wavelength(s). They are further arranged to be suspended in the fluid (200). On the other hand, the system comprises an optical detector (102) of at least a portion of the spectrum of the totally reflected wave (20), said portion including said at least one resonant wavelength(s).

Abstract: A cartridge (110) and a method process target components (T1, T2) of a sample fluid, for example the detection of cardiac markers in blood. The cartridge (110) includes a reaction chamber (114) with a hydrophilic reaction surface (115). A physical barrier (116,118) on the reaction surface (115), for example a protrusion, at least partially borders an investigation region (117,117?) which includes capture probes (CP1, CP2) that specifically bind to target components (T1, T2) of the sample fluid.

Abstract: The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.

Abstract: The invention relates to a cartridge (110) and a method for the processing of target components (T1, T2) of a sample fluid, for example the detection of cardiac markers in blood. The cartridge (110) comprises a reaction chamber (114) with a hydrophilic reaction surface (115). A physical barrier (116,118) on the reaction surface (115), for example a protrusion, at least partially borders an investigation region (117,117?) which comprises capture probes (CP1, CP2) that specifically bind to target components (T1, T2) of the sample fluid.

Abstract: A method of determining a presence of target molecules in a body fluid (16) with cells comprises the steps of: lysing cells in the body fluid (16) for generating a lysate (17); mixing the lysate (16) with particles (36) with first detector molecules; binding target molecule to the particles through first detector molecules; exposing a substrate (38) with second detector molecules to the lysate (17); binding target molecules to the substrate through second detector molecules; detecting particles (36) attached to the substrate (36).

Abstract: The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.

Abstract: A biosensor device (10) for detecting of molecules of an analyte in a sample (23) comprising: an identification element (18) comprising at least one self assembling monolayer (26) having a first surface, a transducer element (20) comprising a metal electrode (34) for receiving an electric signal from the reaction of the molecules in the sample with the at least one self assembling monolayer (26), and at least one electronic element (14, 16) for receiving the electric signal from the transducer element (20), for processing, and/or storing the electric signal. The at least one self-assembling monolayer (26) comprises at least one carboxylic acid-group for coupling the at least one self-assembling monolayer (26) to the surface (32) of the metal electrode (34).