Patents by Inventor Joy L. Silen

Joy L. Silen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
    Patent number: 6455288
    Abstract: The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine.
    Type: Grant
    Filed: April 8, 1993
    Date of Patent: September 24, 2002
    Assignees: Dade Behring Marburg GmbH, Genencor International, Inc.
    Inventors: Edward Benjamin Jakobovits, Joy L. Silen, Mark J. Levy, Thomas C. Goodman, Martin Becker, Edwin F. Ullman, Robert M. Caldwell, Richard R. Bott, Christopher Charles Barnett
  • Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
    Patent number: 6090567
    Abstract: Methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine.
    Type: Grant
    Filed: May 22, 1995
    Date of Patent: July 18, 2000
    Assignee: Behringwerke AG
    Inventors: Edward Benjamin Jakobovits, Joy L. Silen, Mark J. Levy, Thomas C. Goodman, Martin Becker, Edwin F. Ullman, Robert M. Caldwell, Richard R. Bott, Christopher Charles Barnett
  • Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
    Patent number: 6033890
    Abstract: The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine.
    Type: Grant
    Filed: May 22, 1995
    Date of Patent: March 7, 2000
    Assignee: Behring Diagnostics GmbH
    Inventors: Edward Benjamin Jakobovits, Joy L. Silen, Mark J. Levy, Thomas C. Goodman, Martin Becker, Edwin F. Ullman, Robert M. Caldwell, Richard R. Bott, Christopher Charles Barnett