Abstract: A bio-nanocompound incorporating metal oxide particles as substrate, an amino organosilane as linker, a dialdehyde as crosslinking agent and a nucleation agent that utilizes ice nucleating activity to create ice on low energy demand at temperatures down to 0° C. and extend cold chains without increasing energy consumption, wherein even the first application is capable of freezing to different shapes and volumes; a method for producing the bio-nanocompound by self-assembly technique, by mixing the substrate, immobilizing the linker on the substrate, immobilizing the crosslinker on the linker and immobilizing by covalent bonding nucleating agent on the crosslinker; and a coolant having the bio-nanocompound.