Abstract: A control and feedback technique to determine the proper location of powder control temperature regions includes detecting solid parts having specified shape and thermal characteristics. The solid parts are generated by a 3D printer. The solid parts include powder material fused on a layer by layer basis. A powder control temperature region is selected in each layer of powder material, wherein the region is located in unfused powder material adjacent to the solid parts, and wherein the region is selected based on characteristics of the shape of a build area and thermal imaging associated with any of a structural formation of the solid parts adjacent to the region, and an amount of thermal energy radiated by the solid parts adjacent to the region. The amount of thermal energy to cause fusing of the powder material on each subsequent layer is modified based on location of the powder control temperature region.

Abstract: Disclosed is an immunogenic formulation that induces protection against Shiga Toxin-Producing Escherichia coli (STEC), the formulation comprising: one or more chimeric proteins derived wholly or in part from OmpT and Hes proteins of STEC; and at least one physiologically or pharmaceutically acceptable excipient, carrier and/or diluent.

Abstract: A control and feedback technique to determine the proper location of powder control temperature regions includes detecting solid parts having specified shape and thermal characteristics. The solid parts are generated by a 3D printer. The solid parts include powder material fused on a layer by layer basis. A powder control temperature region is selected in each layer of powder material, wherein the region is located in unfused powder material adjacent to the solid parts, and wherein the region is selected based on characteristics of the shape of a build area and thermal imaging associated with any of a structural formation of the solid parts adjacent to the region, and an amount of thermal energy radiated by the solid parts adjacent to the region. The amount of thermal energy to cause fusing of the powder material on each subsequent layer is modified based on location of the powder control temperature region.