Abstract: The present invention provides an aluminum alloy for lithographic sheet including about 0.05 wt % to about 0.25 wt % Si; about 0.25 wt % to about 0.4 wt % Fe; less than or equal to about 0.04 wt % Cu; less than or equal to about 0.25 wt % Mn; 0.31 wt % to 0.35 wt % Mg; less than or equal to about 0.03 wt % Zn; less than or equal to about 0.03 wt % Ti; and incidental impurities. Another aspect of the invention is a method of processing a lithographic sheet including the steps of providing an aluminum sheet; contacting the aluminum sheet with an electrolyte bath; and applying a current having a non-sinusoidal wave form with a constant peak voltage to said electrolyte bath.

Abstract: An isolated DNA sequence that encodes a peptide with CPC-acetylhydrolase enzymatic activity is disclosed. Also, a method of expressing CPC-acetylhydrolase activity that includes the steps of (a) providing a microorganism that is susceptible to transformation with the isolated DNA sequence and that, upon transformation, expresses the CPC-acetylhydrolase activity encoded by the sequence, and (b) transforming the microorganism with the DNA sequence to cause expression of the CPC-acetylhydrolase activity in the microorganism is disclosed. Also, a method for producing a microorganism with increased capacity to aid in the production of cephalosporin, which method includes the steps of (a) providing a microorganism that has CPC-AH activity by virtue of expression of the DNA sequence or of a fragment thereof coding for the CPC-acetylhydrolase enzyme activity; and (b) inactivating the activity by disrupting expression of the DNA sequence is disclosed.

Abstract: Novel methods and compositions are provided for the enhanced production of .beta.-lactam antibiotics. The process is exemplified by the production of penicillin. In addition, the P. chrysogenum and A. chrysogenum .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase genes have been isolated and sequenced. Also methods are provided for the production of .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase using recombined DNA techniques.

Abstract: There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptide, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.