Patents by Inventor Jue Ruan

Jue Ruan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Sequencing library, and preparation and use thereof
    Patent number: 11702690
    Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.
    Type: Grant
    Filed: February 23, 2018
    Date of Patent: July 18, 2023
    Assignee: Agricultural Genomics Institute at Shenzhen China Academy of Agricultural Sciences
    Inventors: Jue Ruan, Kaile Wang
  • Sequencing library, preparation method and use thereof
    Patent number: 10718015
    Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: July 21, 2020
    Assignee: Beijing Institute of Genomica, Chinese Academy of Sciences
    Inventors: Jue Ruan, Kaile Wang, Chung-I Wu, Xuemei Lu
  • PLASMID LIBRARY COMPRISING TWO RANDOM MARKERS AND USE THEREOF IN HIGH THROUGHPUT SEQUENCING
    Publication number: 20200131504
    Abstract: Provided is a plasmid library comprising a DNA insertion site and two barcode sequences located upstream and downstream of the site. The combinations of two barcode sequences of any two plasmids selected from the library are different. Also provided is a method for high-throughput paired-end sequencing of an inserted DNA using the plasmid library.
    Type: Application
    Filed: March 24, 2015
    Publication date: April 30, 2020
    Inventors: Xiao LIU, Zhichao XU, Xiaolin WEI, Zhongyi WU, Jue RUAN
  • SEQUENCING LIBRARY, AND PREPARATION AND USE THEREOF
    Publication number: 20190078157
    Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.
    Type: Application
    Filed: February 23, 2018
    Publication date: March 14, 2019
    Inventors: Jue Ruan, Kaile Wang
  • SEQUENCING LIBRARY, PREPARATION METHOD AND USE THEREOF
    Publication number: 20160362735
    Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.
    Type: Application
    Filed: December 5, 2014
    Publication date: December 15, 2016
    Applicant: Beijing Institute of Genomics, Chinese Academy of Science
    Inventors: Jue Ruan, Kaile Wang, Chung-I Wu, Xuemei Lu