Abstract: A fabric includes mutually transverse thread systems, with at least one of the thread systems including a differential shrinkage yarn C. The shrinkage yarn C has at least one effect component A that irreversibly elongates itself upon heat treatment, and at least one shrinkage component B that shortens itself upon heat treatment. The components A and B are bound together by nodes, wherein the number (y) of nodes per meter in the yarn C is predetermined as a function of the yarn count (x) of the transverse thread system so that the number (y) of nodes exceeds a minimum value and increases proportionally above the minimum value as a function of the yarn count (x).

Abstract: The invention relates to an oligonucleotide which serves as a nucleic acid probe comprising a loop section with a loop sequence complementary to the target sequence of the nucleic acid molecule; a stem section arranged on both ends of the loop section for hybridizing with each other for closing the oligonucleotides thereby forming a loop, and wherein one of the stem sections is labeled with fluorescent color, such that when the loop section hybridizes with the target sequence of the nucleic acid, the oligonucleotide opens and the distance between the fluorophore and a quencher nuleoside increases which thereby prevents quenching of the fluorescence and the strength of fluorescence when measured indicates the presence of the nucleic acid with the target sequence.

Abstract: The invention relates to a device and a method for the detection, especially on line detection, of an amplification of a DNA and/or RNA sequence in a sample. The amplification of the DNA and/or RNA sequence in the sample is evaluated on the basis of scattered-light signal of the sample.

Abstract: In order to carry out optical detection and identification of individual tumor markers in undiluted blood plasma, said tumor markers are marked with fluorescent dyes which are characterized by specific fluorescent lifetimes ranging from 0.5 to 6. The blood plasma is exposed to pulsed diode laser radiation emitted in the 630 to 670 nm wavelength range. The emission wavelengths of the dyes are selected in such a way that they are 10 to 60 nm longer than the wavelength of the diode laser. Blood plasma decay curves are detected using time-correlated single photon counts. Said curves are described by a bi-exponential model wherein it is assumed that a fixed fluorescent lifetime for blood plasma emission is 300 ps and that the marked tumor markers have a variable fluorescent lifetime. The relative amount of fluorescent photons in the marked tumors is determined in relation to the overall number of photons detected. If this value exceeds 0.3, a tumor marker is present in the observed volume.

Abstract: An extended fibre point (1) is coated with photbiotin and irradiated with U.V. light, causing the photobiotin to bind with the fibre pint. The fibre point is then brounght into contact with streptavidin and submerged in a solution containing dyed copies of the DNA molecule to be sequenced. Biotin is also bonded to the 5′ end of the dyed DNA molecules (4A). the biotin coupled to individual DNA molecules binds with the streptavidin at the fibre point. Light is coupled into the fibre to detect the thus obtained bonds. The evanescent field of said light excites a dyed DNA molecule to emit fluorescent light as soon as it binds with the fibre point. When a bond is deteccted, the fibre is immediately remvoded from the solution, and the fibre point is inserted into a microcapillary (5). The microcapillary is filled with a buffer solution containing exonucleases which decompose the DNA colecule base after base.

Abstract: A method for the enzymatic sequencing of DNA is disclosed which uses dideoxythio-nucleotides or dideoxyamino-nucleotides as terminators that are labeled with one or two fluorescent dyes coupled to the terminators either before or after polymerization. The dye labeled DNA fragments are separated in a single lane of a separation system and then the terminal bases of each of the fragments are identified by measuring the fluorescent lifetime of the dye attached to the terminators.

Abstract: In a method for the application of cuts, perforations and/or microlesions to biological material, such as cell agglomerates, individual cells or chromosomes, by means of laser pulses fed into a microscope, the laser pulses are focussed to a cross-section which is limited by diffraction phenomena, and the nth order diffraction maxima (n=1, 2, 3, . . . ) of the laser pulses are used to apply the cuts.