Abstract: The present invention is an automated separation system comprised of a liquid handling device and one or more pipette tip columns, wherein the liquid handling device is equipped with two or more nozzles arranged to respectively receive a pipette tip column comprising separation media. Further, the system includes means for applying either high positive or negative pressure to the column inside chamber above the column bed, enabling aspiration and dispensing into and out of each pipette tip column. To enable sealing to the pressures used to move liquid through the column bed without inadvertently ejecting the column from the nozzle during the separation process, each nozzle has been provided with at least one annular protrusion arranged to engage with the inside of a substantially evenly tapered pipette tip column, without any corresponding recess. Each nozzle may be provided with slide ejector to enable the removal of a pipette tip column.

Abstract: The present invention relates to a pre-packed chromatography cartridge (10) suitable for flash chromatography. The chromatography cartridge (10) comprises a barrel (20) having one end sealed with a cap (40), a thread (23) arranged on the outer cylindrical surface of the barrel (20) and engaged to a mating thread (43) on the inner cylindrical surface of the cap (40). The barrel is provided with at least one protrusion (24) arranged on the outer cylindrical surface and below the thread (23) in the direction from the end sealed by the cap (40). The protrusion (24) extends essentially radially outwards from the outer cylindrical surface of the barrel (20). The cap (40) is provided with a cylindrical flange (44) arranged below the thread (43) of the cap (40). A locking member (46) is arranged on the flange (44) and extends essentially radially inwards from the inner cylindrical surface of the flange (44).

Abstract: The present invention relates to a pre-packed chromatography cartridge (10) suitable for flash chromatography. The chromatography cartridge (10) comprises a barrel (20) having one end sealed with a cap (40), a thread (23) arranged on the outer cylindrical surface of the barrel (20) and engaged to a mating thread (43) on the inner cylindrical surface of the cap (40). The barrel is provided with at least one protrusion (24) arranged on the outer cylindrical surface and below the thread (23) in the direction from the end sealed by the cap (40). The protrusion (24) extends essentially radially outwards from the outer cylindrical surface of the barrel (20). The cap (40) is provided with a cylindrical flange (44) arranged below the thread (43) of the cap (40). A locking member (46) is arranged on the flange (44) and extends essentially radially inwards from the inner cylindrical surface of the flange (44).

Abstract: A flow control device, a system and a method are disclosed. The flow control device comprises a displaceable means (101) having a direction of displacement and being displaceable between a first position and a second position. The flow control device further comprises a body (110) comprising a first cavity (102) in fluid contact with a first side of the displaceable means (102) forming a first volume. The first cavity further comprising a first inlet (103), a primary outlet (104) with a primary flow resistance and arranged essentially parallel with the displacement direction, wherein the primary outlet comprises an inlet opening (105) facing the displaceable means at a first distance (106) from the displaceable means in the direction of displacement, a secondary outlet (107) with a secondary flow resistance, wherein the displaceable means is displaceable between a first position and a second position.

Abstract: A chromatography column comprises a tubular member (1) having an open inlet end and an outlet end and being adapted to receive a packing material, and an end piece (2) inserted through the inlet end of the tubular member (1). The end piece (2) comprises a generally planar inner end portion 4, and a rounded outer end portion (5), adapted to accommodate pressure forces acting on the inner end. The end piece (2) is fixed to the inner surface of the tubular member (1) by a weld- or bond-forming joining technique based on non-contact energy transfer, preferably laser welding. A method for manufacturing a pre-packed chromatography column is also disclosed.

Abstract: A chromatography column comprises a tubular member (1) having an open inlet end and an outlet end and being adapted to receive a packing material, and an end piece (2) inserted through the inlet end of the tubular member (1). The end piece (2) comprises a generally planar inner end portion 4, and a rounded outer end portion (5), adapted to accommodate pressure forces acting on the inner end. The end piece (2) is fixed to the inner surface of the tubular member (1) by a weld- or bond-forming joining technique based on non-contact energy transfer, preferably laser welding. A method for manufacturing a pre-packed chromatography column is also disclosed.