Abstract: The present invention relates to an extracellular vesicle (EV)-based nucleic acid composition or vaccine (EV-NAV), comprising EVs loaded with polynucleotides each encoding, e.g., the SARS-CoV-2 spike protein, and polynucleotides each encoding, e.g., SARS-CoV-2 nucleocapsid protein, wherein said polynucleotides are designed to be simultaneously expressed, and to induce a humoral immune response and/or a cellular immune response, in a subject. The present invention also relates to compositions and methods for the design, preparation, manufacture, formulation, and therapeutic or prophylactic use of said EV-NAVs, e.g., exosomes loaded with mRNAs encoding multiple surface and cytoplasmic antigens derived from, e.g., SARS-CoV-2, to elicit strong humoral and cellular immune responses.

Abstract: A wafer stacking method includes the following steps. A first wafer is provided. A second wafer is bonded to the first wafer to form a first wafer stack structure. A first edge defect inspection is performed on the first wafer stack structure to find a first edge defect and measure a first distance in a radial direction between an edge of the first wafer stack structure and an end of the first edge defect away from the edge of the first wafer stack structure. A first trimming process with a range of a first width is performed from the edge of the first wafer stack structure to remove the first edge defect. Herein, the first width is greater than or equal to the first distance.

Abstract: A wafer processing method including following steps is provided. A release layer is formed on a first wafer. An adhesive layer is formed on a second wafer. One of the first wafer and the second wafer is a device wafer. The device wafer includes a valid die region and a trimming region. A handler is applied to place the first wafer on the second wafer, so that the release layer and the adhesive layer are bonded to each other, and the adhesive layer completely covers the valid die region. During the process of placing the first wafer on the second wafer, the handler directly moves the first wafer.

Abstract: Coronavirus egress is mediated by lysosomal exocytosis. It is demonstrated herein that the D614G mutation enhances Spike trafficking to lysosomes and the lysosomal accumulation of newly synthesized virus particles, augments Spike-mediated disruption of endomembrane homeostasis, and causes a 3-fold reduction in cell surface Spike expression. Moreover, it is shown that the D614G mutation is an intragenic suppressor of the 12 nucleotide-long furin cleavage site (FCS) insertion, restoring Spike trafficking to lysosomes and TMPRSS2-independent infectivity, both of which had been impaired by the prior FCS insertion mutation. This data identifies enhanced lysosomal sorting as the earliest known manifestation of the D614G mutation, have implications for virus evolution, immunity, and vaccine design, and support a lysosomal model of coronavirus biogenesis and entry.

Abstract: The present disclosure provides methods for the detection of target specific antibodies in samples. The methods include the detection of pathogen-specific antibodies, such as SARS-CoV-2 specific antibodies. Also included is a kit for use for the detection of pathogen specific antibodies in a sample. In one aspect, the disclosure provides a method of detecting the presence of a target specific antibody in a sample by (i) contacting the sample with a test cell comprising one or more exogenous nucleic acid sequences encoding one or more target proteins; and (ii) detecting the presence of the target specific antibody in the sample by contacting the immune complex of (i) with an anti-immunoglobulin (Ig) antibody, and detecting the anti-immunoglobulin (Ig) antibody, thereby detecting the presence of a target specific antibody.

Abstract: The present invention relates to engineered selectable marker proteins for recombinant protein expression, as well as novel expression vector designs for achieving high-level recombinant protein expression, and cells transfected therewith as a platform technology for producing extracellular vesicle-based therapeutic or prophylactic compositions, wherein one or more recombinant proteins of interest are displayed on the surface of the extracellular vesicles. As an example, the present invention relates to a virus-like article composition comprising such extracellular vesicles displaying one or more antigens configured to induce immune responses against SARS-CoV-2.

Abstract: A biodegradable composite includes an organic matter and a porous material, wherein the organic matter has viable bacteria, and a total plate count of the organic matter is greater than or equal to 104 CFU/g. The organic matter accounts for 40% to 80% of a weight of the biodegradable composite. The porous material accounts for 20% to 60% of the weight of the biodegradable composite. The biodegradable composite could instantly remove unpleasant odor and accelerate a decomposition process to form compost. A product containing the biodegradable composite is provided as well.

Abstract: A process for forming a two layer plastic device with textures or drawings thereon and a structure formed by the structure are provided. An inner bottom layer has textures or drawings on the surface thereof which includes at least one enclosing space. A through hole is formed on the enclosing space so that the masking material can be filled into the space between the outer mold and the inner mold. Then the masking material enters into the enclosing space through the through hole. As a result, only one mold (the outer mold) is necessary for forming the masking layer, while in the prior art, two molds are needed. The whole process can be completed mechanically without any manual operation. A color of the masking layer may be different from that of the inner bottom layer so that the textures or drawings clearly exposes on the surface of the masking layer.

Abstract: A process for forming a two layer plastic device with textures or drawings thereon and a structure formed by the structure are provided. An inner bottom layer has textures or drawings on the surface thereof which includes at least one enclosing space. A through hole is formed on the enclosing space so that the masking material can be filled into the space between the outer mold and the inner mold. Then the masking material enters into the enclosing space through the through hole. As a result, only one mold (the outer mold) is necessary for forming the masking layer, while in the prior art, two molds are needed. The whole process can be completed mechanically without any manual operation. A color of the masking layer may be different from that of the inner bottom layer so that the textures or drawings clearly exposes on the surface of the masking layer.

Abstract: An electrical connector used for bearing a chip module includes an insulating body, terminals arranged in the insulating body, a carrying member, for carrying the chip module to the insulating body, disposed at one side of the insulating body and contacting with the terminals, a pressing plate for pressing the carrying member or the chip module, and an elastic member having at least two limiting portions. The pressing plate and the carrying member are separately disposed at two adjacent sides on the periphery of the insulating body. The two limiting portions define a pivoting space, and the carrying member is pivotally connected to the pivoting space. The elastic member and the carrying member are disposed at the same side of the insulating body, the elastic member has a buffer portion, and the buffer portion abuts against the carrying member and is disposed between the insulating body and the carrying member.

Abstract: An electrical connector used for carrying a chip module includes an insulating body with multiple terminals arranged in the insulating body, a carrying member pivoted to the insulating body and used for carrying the chip module to the insulating body, and a pressing plate located on one side of the insulating body and used for pressing the carrying member or the chip module. The chip module contacts with the multiple terminals. The carrying member is directly pivoted to the insulating body. Each of two ends of a pivoting side of the carrying member is provided with a pivot shaft. Fixing holes are formed on the outer side of the insulating body. The pivoting shafts are pivoted into the fixing holes, so that the carrying member is rotatingly pivoted to the insulating body without the assistance of a pivoting device for pivoting the carrying member.

Abstract: An electrical connector used for bearing a chip module includes an insulating body, terminals arranged in the insulating body, a carrying member, for carrying the chip module to the insulating body, disposed at one side of the insulating body and contacting with the terminals, a pressing plate for pressing the carrying member or the chip module, and an elastic member having at least two limiting portions. The pressing plate and the carrying member are separately disposed at two adjacent sides on the periphery of the insulating body. The two limiting portions define a pivoting space, and the carrying member is pivotally connected to the pivoting space. The elastic member and the carrying member are disposed at the same side of the insulating body, the elastic member has a buffer portion, and the buffer portion abuts against the carrying member and is disposed between the insulating body and the carrying member.

Abstract: An electrical connector used for carrying a chip module includes an insulating body having terminals arranged in the insulating body and used for contacting the chip module, a carrying member used for carrying the chip module to the insulating body, a pressing plate used for pressing the carrying member or the chip module, and a positioning member pivoted to the carrying member through a pivoting device. The pivoting device includes a pivoting shaft and at least one elastic portion. The elastic portion includes a first elastic section and at least two limiting portions being elastic and enclosing a pivoting space. The pivoting shaft is pivoted to the pivoting space. When the electrical connector is opened, the first elastic section springs back to remove the chip module from the insulating body without damaging the terminals, so as to align and contact the chip module with the terminals without tool.

Abstract: An extract of Asplenium nidus L. includes pyropheophorbide a methyl ester (C34H36N4O3), pheophorbide a methyl ester (C35H36N4O5), 1-linleoyl-3-linolenoyl-glycerol (C39H66O5), 1-linleoyl-2,3-dipalmitoyl-rac-glycerol (C53H98O6) and 1,3-dipalmitoyl-sn-glycerol (C35H68O5). The extract of Asplenium nidus L. is obtained by using a method including steps of: solvent extraction, using an solvent to extract an Asplenium nidus L. sample and to obtain an extract, with a w/v ratio between the solvent and the Asplenium nidus L. sample being 50˜60 mg/ml; and column chromatography, fractionating the extract with water and ethanol as eluent to obtain several fractions including fraction a to fraction i, and further evaporating and lyophilizing fraction b, c, d or their combination to obtain an extract of Asplenium nidus L.

Abstract: A method of damaging cell structure of an aquatic substance includes: providing an aquatic substance raw material, where the aquatic substance raw material includes an aquatic substance; adjusting a water content in the aquatic substance raw material to form an aquatic substance slurry to be processed; placing the aquatic substance slurry to be processed in a pressure container; introducing a compressed gas into the pressure container to enable the compressed gas and the water in the aquatic substance slurry to be processed to form an acidic fluid, and making the cell structure of the aquatic substance hydrolyzed and damaged by the acidic fluid; and performing a depressurizing step to separate the compressed gas.

Abstract: A liquid ornament is revealed. The liquid ornament includes a closed container and a film sheet. A liquid ornament is received in the closed container and is displayed. The liquid ornament consists of combinations of at least two of a first liquid, a second liquid and at least one ornament. The film sheet is formed by a first end surface and a second surface opposite to the first end surface. The first end surface is attached to a connection part of the closed container while the second end surface is attached to a surface of a solid object.

Abstract: A dynamic liquid ornament including a transparent container, an ornamental object and a base is revealed. The transparent container has an opening on a bottom thereof, at least one cavity filled with liquid, and a neck portion on the bottom of the cavity. The size of the ornamental object is larger than the neck portion of the cavity. Thus the ornamental object is compressed and elastically-deformed to pass through the opening of the transparent container, the neck portion of the cavity, and suspend in the liquid. A first magnet is disposed on the bottom of the ornamental object. The base mounted with a rotation device therein is connected to the bottom of the transparent container. The rotation device is connected to a second magnet for driving the second magnet to rotate. The first magnet is affected by the second magnet to make the ornamental object rotate.

Abstract: An electric inversion table includes a base, a bench and a driving device. The bench is disposed on the base and is rotated between a horizontal position and a vertical position. The driving device connects the bench to the base for driving the bench to rotate between the horizontal position and the vertical position. When power failure occurs or a motor of the driving device is out of order, users can release locked status by a clutch of the driving device so as to turn the bench from the vertical position back to the horizontal position safely by the body weight.

Abstract: A work output device is revealed. The work output device includes two motor rotors respectively arrange at two ends of a horizontal main rotating shaft. An outer transmission shaft of each motor rotor starts rotating after the power is turned on. The rotating outer transmission shaft drives the main rotating shaft to rotate in one direction through a speed change gear mechanism. The motor rotors are synchronously driven to rotate around the main rotating shaft and move from a lower position to a higher position. At the moment, the power is turned off and the motor rotors turn back from the higher position to the lower position with the help of gravity. Thus a cycle of repetitive up-and-down half circle rotation is completed. Moreover, the main rotating shaft drives a work output shaft to rotate by a torque controller.

Abstract: An extract of Asplenium nidus L. includes pyropheophorbide a methyl ester (C34H36N4O3), pheophorbide a methyl ester (C35H36N4O5), 1-linleoyl-3-linolenoyl-glycerol (C39H66O5), 1-linleoyl-2,3-dipalmitoyl-rac-glycerol (C53H98O6) and 1,3-dipalmitoyl-sn-glycerol (C35H68O5). The extract of Asplenium nidus L. is obtained by using a method including steps of: solvent extraction, using an solvent to extract an Asplenium nidus L. sample and to obtain an extract, with a w/v ratio between the solvent and the Asplenium nidus L. sample being 50˜60 mg/ml; and column chromatography, fractionating the extract with water and ethanol as eluent to obtain several fractions including fraction a to fraction i, and further evaporating and lyophilizing fraction b, c, d or their combination to obtain an extract of Asplenium nidus L.