Abstract: An Antrodia cinnamomea cultivation method sequentially comprises the following steps: step 1, obtaining a nutrient solution by mixing in proportion, wherein ingredients of the nutrient solution comprises Cinnamomum kanehirai extracts, carbon sources and nitrogen sources, the nutrient solution is placed in a porous carrier, the porous carrier has a peripheral surface and multiple micropores recessed into the peripheral surface, the nutrient solution is able to infiltrate into the micropores to form a nutrient layer on the peripheral surface and inner peripheries of the micropores; step 2, performing a membrane treatment on the porous carrier to form a membrane on the nutrient layer; step 3, sterilizing the porous carrier; and step 4, growing Antrodia cinnamomea strains on the membrane on the porous carrier, and then placing the porous carrier in a sealed environment for cultivation at constant temperature and humidity.