Patents by Inventor Julia Fletcher
Julia Fletcher has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150267209Abstract: The present disclosure describes a novel recombinant gene expression system and a method of its use. The recombinant gene expression system is adapted to enable a user to monitor the successful expression of a protein in real time by allowing a small portion (e.g., less than 10%) of the target protein to be expressed as a fusion with a readily detectable reporter. The recombinant gene expression system further allows a user to select a clone that exhibits high, medium or low expression by providing a random ribosomal binding site having the nucleotide sequence N4R6NX, where N is A, T, G, or C, where R is A or G, and where x is an integer from 6 to 11 (SEQ ID NO: 1) upstream of the cloned gene. By selecting a clone exhibiting a desired relative level of reporter gene expression, a user can tailor the expression level of the desired protein.Type: ApplicationFiled: October 22, 2013Publication date: September 24, 2015Inventors: Jeffrey Rogers, Julia Fletcher, Kevin Lowitz
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Publication number: 20120190064Abstract: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.Type: ApplicationFiled: January 23, 2012Publication date: July 26, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wieslaw Antoni KUDLICKI, Shiranthi Keppetipola, Julia Fletcher, Ashley Elaine Getbehead, Federico Katzen, Laura Vozza-Brown
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Publication number: 20110195450Abstract: In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.Type: ApplicationFiled: July 30, 2010Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: WIESLAW KUDLICKI, JULIA FLETCHER, FEDERICO KATZEN
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Publication number: 20110104781Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: September 24, 2010Publication date: May 5, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: FEDERICO KATZEN, JULIA FLETCHER, WIESLAW KUDLICKI, JOSEPH BEECHEM, LILIN WANG
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Publication number: 20100233782Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: October 9, 2009Publication date: September 16, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang
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Publication number: 20090209032Abstract: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.Type: ApplicationFiled: September 15, 2008Publication date: August 20, 2009Applicant: INVITROGEN CORPORATIONInventors: Wieslaw Antoni Kudlicki, Shiranthi Keppetipola, Julia Fletcher, Ashley Elaine Getbehead, Federico Katzen, Laura Vozza-Brown
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Publication number: 20090161828Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: December 11, 2008Publication date: June 25, 2009Applicant: LIFE TECHNOLOGIESInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang
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Publication number: 20080248565Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: February 29, 2008Publication date: October 9, 2008Applicant: INVITROGEN CORPORATIONInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang
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Publication number: 20070117179Abstract: In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.Type: ApplicationFiled: September 27, 2006Publication date: May 24, 2007Applicant: INVITROGEN CORPORATIONInventors: Wieslaw Kudlicki, Julia Fletcher, Federico Katzen
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Publication number: 20060211083Abstract: Provided herein are products and processes for efficiently synthesizing biomolecules in vitro using cell-free extracts derived from mammalian cells and insect cells. In an embodiment, provided is a process for preparing a cell-free extract from insect cells and mammalian cells that efficiently synthesizes post-translationally modified target proteins (e.g., glycosylated target proteins). In some embodiments, a ribonucleic acid is synthesized in vitro that comprises a cap, a 5? untranslated region comprising an 18S rRNA binding ribonucleotide sequence, and a target ribonucleotide sequence. It has been determined that such ribonucleic acids result in efficient in vitro synthesis of a target protein using cell-free extracts derived from non-rabbit mammalian cells and insect cells.Type: ApplicationFiled: January 20, 2006Publication date: September 21, 2006Inventors: Federico Katzen, Wieslaw Kudlicki, Julia Fletcher
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Publication number: 20060110788Abstract: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.Type: ApplicationFiled: October 3, 2005Publication date: May 25, 2006Applicant: Invitrogen CorporationInventors: Wieslaw Kudlicki, Shiranthi Keppetipola, Julia Fletcher, Ashley Getbehead, Federico Katzen, Laura Vozza-Brown
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Publication number: 20060084136Abstract: The present invention relates to in vitro protein synthesis (IVPS) systems, particularly such systems using suppressor tRNAs and rare codon tRNAs to extend translation of an open reading frame into fusion protein elements, thereby generating fusion proteins in vitro. The invention also provides methods, compositions and kits using the IVPS systems of the invention, and proteins produced using the methods, compositions, kits and IVPS systems of the invention.Type: ApplicationFiled: July 14, 2005Publication date: April 20, 2006Applicant: Invitrogen CorporationInventors: Wieslaw Kudlicki, Julia Fletcher, Federico Katzen, Robert Bennett