Abstract: A method and a system of determining activity of a target enzyme of a sample is described. The method comprises bringing the sample in physical contact with a substrate for the target enzyme, incubating the sample with the substrate for an actual incubating time, determining a parameter value associated to enzymatic actions of the target enzyme involving the substrate, correlating the actual incubation time for the sample to at least two sets of reference data, determining a best fit standard curve correlated to the actual incubation time for the sample, and correlating the determined parameter value to the best fit standard curve and determine the target enzyme activity. Each of the at least two sets of reference data comprises data representing a standard curve for the parameter associated to the enzymatic actions of the target enzyme involving the substrate as a function of enzyme activity and correlated to an incubation time.

Abstract: A method of determining activity of a target enzyme of a grain material is described. The method comprises providing a fluid extract sample of the grain material using a preselected extraction procedure, providing a dyed and/or chromogenic substrate for the target enzyme, subjecting the fluid extract sample to the substrate for a preselected incubating time, and determining the target enzyme activity of the grain material. The extraction time is relatively short while still obtaining high accuracy.

Abstract: The invention relates to an enzyme activity device suitably for determination of biopolymer enzyme degrading activity in a liquid sample, said enzyme activity device comprising a biopolymer substrate and a solid support structure supporting said biopolymer substrate wherein said biopolymer substrate comprises a dyed and water insoluble xerogel comprising a network of cross-linked biopolymers. The invention also relates to an enzyme activity assay and a method of determining enzyme activity of a sample.