Abstract: The present invention provides for a composition comprising a purified or isolated Hyde1 gene product, or a functional fragment thereof; a modified host cell capable of expressing a Type VI secretion system (T6SS), Hyde1, and/or Hyde2, or a functional fragment thereof; a method of treating a disease caused all or in part by a bacterial cell, comprising administering a composition of the present invention to a subject in need thereof; and a method to limit or reduce growth of a pathogenic bacteria in an environment, comprising: introducing a non-pathogenic bacterial capable of expressing a Type VI secretion system (T6SS), Hyde1, and/or Hyde2, or functional fragment thereof, to an environment.

Abstract: The invention relates to a method for spatially manipulating a microscopic object including providing a cantilever (12) having a tip with an opening (19) and a microchannel (15) extending through the cantilever (12) in its longitudinal direction. A suspension means is provided for holding the cantilever (12) and spatially moving the cantilever along a predetermined spatial path. A pressurizing means is provided for applying a predetermined pressure to the microchannel (15) and the cantilever (12) is moved with its tip to the microscopic object to be spatially manipulated. At least a part of the microscopic object is picked up with said cantilever (12) by reducing the pressure within the microchannel (15) relative to the pressure outside the tip of the cantilever (12). The microscopic object is then moved along a predetermined spatial path by means of the cantilever (12).

Abstract: The invention relates to a method for spatially manipulating a microscopic object (20,23,33), said method comprising the steps of providing a cantilever (12) having a tip with an opening (19) and a microchannel (15) extending through the cantilever (12) in its longitudinal direction, said microchannel (15) being fluidly connected to the opening (19) at the tip of the cantilever; providing suspension means for holding the cantilever (12) and spatially moving the cantilever along a predetermined spatial path; providing pressurizing means for applying a predetermined pressure to the microchannel (15) within the cantilever; moving the cantilever (12) with its tip to the microscopic object (20,23,33) to be spatially manipulated, such that the opening (19) of the tip is adjacent to the microscopic object (20,23,33); picking up, with said cantilever (12), a part of the microscopic object (20,23,33) or the microscopic object (20,23,33) as a whole by reducing the pressure within the microchannel (15) relative to the p