Abstract: The present invention provides a method of identifying an amino acid position of a DNA modifying enzyme for insertion of a heterologous DNA binding domain (DBD), the method comprising the steps of providing a library of DNA modifying enzymes, wherein the members of the library comprise heterologous amino acid sequence insertions throughout the DNA modifying enzyme; identifying those DNA modifying enzymes of the library that have DNA modifying activity; and identifying the position of the insertion in those DNA modifying enzymes identified in the previous step. The present invention further pertains to methods of producing DNA modifying enzymes comprising an inserted heterologous DBD, to methods for modifying a nucleic acid sequence in a cell, and to methods for evolving a DNA binding domain on desired target sequences. Further provided are nucleic acid sequences encoding such DNA modifying enzymes and DNA binding domains, respective vectors and host cells.

Abstract: A method for calibrating measuring channels of an analysis system for lab-on-a-chip cartridges by way of a calibration cartridge is disclosed.

Abstract: Provided herein are dental compositions and methods for use in dental cementation applications.

Abstract: An apparatus and method for detecting the type, presence or amount of bacteria in a sample. The apparatus and method involve staining a liquid sample with a fluorescent stain corresponding to bacteria and imaging one or more portions of the sample at a selected set of sample Z-axis heights, X-Y coordinates, or both, within the sample after a predetermined time to allow for non-bacterial components in the sample to settle out of solution. A calibration method for correlating the concentration or amount of bacteria in biological samples by different analysis methods including glass slide methods, agar plate colony culture methods and high precision methods.

Abstract: An electrochemical reactor, including: a first magnetic field source; a second magnetic field source; and an electrochemical cell between the first magnetic field source and the second magnetic field source, the electrochemical cell comprising an anode and a cathode, wherein the anode and the cathode are in a channel configured to contain an electrolyte stream comprising an iron-containing feedstock, and wherein the anode and the cathode are configured to contact the electrolyte stream, and wherein the electrochemical reactor is configured to electrochemically reduce at least a portion of the iron-containing feedstock to iron metal at the cathode and in a magnetic field provided by the first magnetic field source, the second magnetic field source, or a combination thereof.

Abstract: A coated textile contains a web based on glass fibres having a back face, a front face and at least one edge. The front face is covered with a coating layer based on silicone and back face is capable of being bonded to a support. The coating layer has a thickness between 5 ?m and 250 ?m and includes at least one inorganic flame retardant compound in a quantity which is sufficient for the coated textile to have a gross calorific value of less than or equal to 3 MJ/kg, the inorganic flame retardant compound having a D50 granule size of less than 50 ?m.

Abstract: A multi-fiber light guide includes: light guiding fibers, each fiber including an elongated glass core; a glass cladding, the cores being surrounded by the cladding to form a rigid and continuous glass element, the cores having a higher refractive index than the cladding such that light can be guided by a total reflection along the cores, which end in two abutting faces of the glass element such that light can be guided along the cores from one abutting face to the other abutting face; and an ion exchange layer at each of the abutting faces, the glass of the cores and the glass of the cladding including alkali ions, which are at least partly exchanged by alkali ions of a higher atomic number within the ion exchange layer at the abutting faces, the exchanged alkali ions within the ion exchange layer imparting a compressive stress at the abutting faces.

Abstract: Computer-implemented methods may include identifying a polymer for decomposition. The method may further include accessing, for an ionic liquid, one or more properties corresponding to the polymer. One or more properties may characterize a reaction between the polymer and the ionic liquid. The method may also include accessing a value of the property using a quantum-mechanical or thermodynamical method. The method may include determining a bond string and position (BSP) representation of a molecule of the ionic liquid. The method may further include determining an embedded representation of the ionic liquid based on the BSP representation. In addition, the method may include generating a relationship between BSP representations of molecules and the one or more properties. The method may also include identifying an ionic liquid as a prospect for depolymerizing the specific polymer based on the relationship. The method may include outputting an identification of the ionic liquid.

Abstract: Provided herein are methods for detecting tumor mutational burden (TMB) in subjects. In one aspect, the methods include determining observed mutational counts from sequence information obtained from nucleic acids in samples from the subjects and determining a tumor fraction and/or a coverage of the nucleic acids to generate sequencing parameters. The methods also include determining an expected mutational fraction and/or an expected distribution of the expected mutational fraction given the sequencing parameters to generate an expected result, and adjusting the observed mutational count given the expected result to generate an adjusted result, thereby detecting the TMB in the subject. Other aspects are directed to methods of selecting customized therapies for treating cancer in subjects, and methods of treating cancer in subjects. Yet other aspects include related systems and computer readable media used to detect TMB in subjects.

Abstract: The present disclosure relates generally to systems and methods for providing reel-spin game enhancement features. A player enrolled with a subscription service server is presented with an opportunity to enhance game features associated with a gaming device after a result of a game played on the gaming device is determined. The gaming device determines that only when the result of the game played corresponds to a predetermined outcome, a respin option becomes available for the player. This option is rendered as an offer for the player to activate a game enhancement feature for a respin during a subsequent play of the reel-spin game. The game enhancement features include holding or locking a symbol in the reels of the reel-spin game, adding a score multiplier, or adding a modified symbol to a particular position or reel, during the respin of the subsequent play of the reel-spin game.

Abstract: An apparatus for replacing at least one tooth of a gear includes an elongate anchoring lip having a laterally extending lip body with at least one anchoring hole extending longitudinally therethrough. The lip body is bounded within a lateral plane by transversely spaced front and rear lip faces and laterally spaced first and second side edges. A tooth block extends longitudinally from a selected one of the front and rear lip faces. The tooth block includes at least one replacement gear tooth extending longitudinally therealong. The tooth block is spaced laterally apart from at least one of the first and second side edges, with at least a portion of the lip body extending laterally between the tooth block and the at least one of the first and second side edges.

Abstract: A computer-implemented method for authenticating an online user includes steps including receiving, from a requestor server in communication with a merchant website, an authentication request message including authentication data collected from a user computing device during an online interaction with the merchant website. The steps also include extracting the authentication data from the authentication request message, and applying a risk-based authentication (RBA) engine to the authentication data to obtain RBA result data including a reason code that includes no more than three bytes of data. The steps further include causing the reason code to be embedded in an authorization request message generated during the online interaction and routed to a decisioning server via a payment network.

Abstract: One example method includes accessing at a Time-Aware Scheduler (TAS) of a Time-Sensitive Networking (TSN) network a plurality of data packets, where each data packet includes a data packet priority that defines an order for transmitting the plurality of data packets by the TSN network; grouping the plurality of data packets into a cluster according to the data packet priorities, the cluster identifying a first TSN network state; applying a first machine-learning model to the cluster to determine a TAS cycle policy that defines a timing schedule for data packet transmission in the TSN network; and providing the TAS cycle policy to components of the TSN network.

Abstract: In various examples, a current claimed set of points representative of a volume in an environment occupied by a vehicle at a time may be determined. A vehicle-occupied trajectory and at least one object-occupied trajectory may be generated at the time. An intersection between the vehicle-occupied trajectory and an object-occupied trajectory may be determined based at least in part on comparing the vehicle-occupied trajectory to the object-occupied trajectory. Based on the intersection, the vehicle may then execute the first safety procedure or an alternative procedure that, when implemented by the vehicle when the object implements the second safety procedure, is determined to have a lesser likelihood of incurring a collision between the vehicle and the object than the first safety procedure.

Abstract: An ear tip is described for retaining a hearing device or a hearing device part in an ear canal of a user wearing the hearing device or the hearing device part. The ear tip includes a sealing element with a sound channel providing for a sound path between a sound inlet opening for receiving an acoustic signal from an output unit of the hearing device or the hearing device part and a sound outlet opening for releasing the acoustic signal to the ear canal of the user wearing the hearing device or the hearing device part, and a first filtering element arranged in the sound channel between the sound inlet opening and the sound outlet opening.

Abstract: Provided herein are Camptothecin Conjugates, Camptothecin-Linker Compounds, Camptothecin Compounds, intermediates thereof, and method of preparing the same. Also provided herein are methods of treating cancer and autoimmune diseases with the Conjugates described herein.

Abstract: A power tool is disclosed and includes a housing and a motor disposed within the housing. The power tool further includes a first blade and a second blade movable relative to the first blade by the motor. During operation, a cutting stroke length of the first blade relative to the second blade is selectively adjustable based on a direction of rotation of the motor.

Abstract: A system and method of creating molten metal droplets is disclosed. The system may be used for 3D printing. The system relies on a change in momentum of the droplet relative to the tip of the metal microwire to cause the droplet to separate from the tip of the metal microwire. The change in momentum can be created by using an oscillating printhead. In other embodiments, a mass strikes the printhead to cause the droplet to separate from the metal microwire. The metal microwire may be heated using a heat source, such as a laser, an induction coil or a plasma arc.

Abstract: An ostomy device includes an implant shaped in a ring and sized for placement in or adjacent to a hole in an abdominal wall, the implant including a surface for contacting a lumen. The implant includes a first part including the surface for contacting the lumen, the first part, when placed in or adjacent to the hole in the abdominal wall, extending in a direction orthogonal to the abdominal wall to support the lumen. The implant includes a region for attaching an ostomy bag.

Abstract: The present disclosure relates to anti-glyco-cMET antibodies and antigen binding fragments thereof that specifically bind to a cancer-specific glycosylation variant of cMET and related fusion proteins and antibody-drug conjugates, as well as nucleic acids encoding such biomolecules. The present disclosure further relates to use of the antibodies, antigen-binding fragments, fusion proteins, antibody-drug conjugates and nucleic acids for cancer therapy.