Patents by Inventor Julian A. J. Hanak

Julian A. J. Hanak has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Plasmid stabilization
    Patent number: 6743780
    Abstract: A system is described which utilizes a novel system of repressor titration for maintenance of a plasmid useful in gene therapy and production of a recombinant protein. The system utilizes a transformed host cell containing a plasmid including an operator susceptible to binding by a repressor expressed in trans, a first chromosomal gene encoding the repressor, and a second chromosomal gene that is functionally associated with an operator and essential for cell growth, wherein the plasmid is present in the cell in sufficient numbers to titrate the repressor such that the essential gene is expressed, thereby permitting cell growth.
    Type: Grant
    Filed: November 12, 1999
    Date of Patent: June 1, 2004
    Assignee: Cobra Biologics Limited
    Inventors: Julian A. J. Hanak, Steven G. Williams, Scott D. Gorman, David J. Sherratt
  • Method of plasmid DNA production and purification
    Patent number: 6503738
    Abstract: A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.
    Type: Grant
    Filed: June 10, 1999
    Date of Patent: January 7, 2003
    Assignee: Cobra Therapeutics Limited
    Inventors: David R. Thatcher, Anthony Hitchcock, Julian A. J. Hanak, Diane L. Varley
  • Method of plasmid DNA production and purification
    Patent number: 5981735
    Abstract: A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.
    Type: Grant
    Filed: February 12, 1997
    Date of Patent: November 9, 1999
    Assignee: Cobra Therapeutics Limited
    Inventors: David R. Thatcher, Anthony Hitchcock, Julian A.J. Hanak, Diane L. Varley
  • Plasmid stabilization
    Patent number: 5972708
    Abstract: A system is described which utilizes a novel system of repressor titration for maintenance of a plasmid useful in gene therapy and production of a recombinant protein. The system utilizes a transformed host cell containing a plasmid including an operator susceptible to binding by a repressor expressed in trans, a first chromosomal gene encoding the repressor, and a second chromosomal gene that is functionally associated with an operator and essential for cell growth, wherein the plasmid is present in the cell in sufficient numbers to titrate the repressor such that the essential gene is expressed, thereby permitting cell growth.
    Type: Grant
    Filed: October 21, 1998
    Date of Patent: October 26, 1999
    Assignee: Cobra Therapeutics Limited
    Inventors: David J. Sherratt, Steven G. Williams, Julian A.J. Hanak